Proteomic analysis reveals mechanisms of mixed wastewater with different N/P ratios affecting the growth and biochemical characteristics of Chlorella pyrenoidosa

Xinrui Mao; Xu Zhou; Xiumin Fan; Wenbiao Jin; Jingjing Xi; Renjie Tu; Mu Naushad; Xuan Li; Huan Liu; Qilin Wang

doi:10.1016/j.biortech.2023.129141

Proteomic analysis reveals mechanisms of mixed wastewater with different N/P ratios affecting the growth and biochemical characteristics of Chlorella pyrenoidosa

Bioresour Technol. 2023 Aug:381:129141. doi: 10.1016/j.biortech.2023.129141. Epub 2023 May 9.

Authors

Xinrui Mao¹, Xu Zhou², Xiumin Fan³, Wenbiao Jin¹, Jingjing Xi¹, Renjie Tu¹, Mu Naushad⁴, Xuan Li⁵, Huan Liu⁵, Qilin Wang⁵

Affiliations

¹ State Key Laboratory of Urban Water Resource and Environment, School of Civil and Environmental Engineering, Harbin Institute of Technology (Shenzhen), Shenzhen, Guangdong 518055, China.
² State Key Laboratory of Urban Water Resource and Environment, School of Civil and Environmental Engineering, Harbin Institute of Technology (Shenzhen), Shenzhen, Guangdong 518055, China. Electronic address: zhouxu@hit.edu.cn.
³ Shenzhen Ecological and Environmental Intelligent Management and Control Center, Shenzhen 518034, China.
⁴ Department of Chemistry, College of Science, King Saud University, P.O. Box 2455, Riyadh, Saudi Arabia.
⁵ Centre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Ultimo, NSW 2007, Australia.

PMID: 37169198
DOI: 10.1016/j.biortech.2023.129141

Abstract

Effects of different nutrient ratios on the biochemical compositions of microalgae and the changes were rarely studied at the molecular level. In this study, the impacts of various nitrogen to phosphorus (N/P) ratios on growing of C. pyrenoidosa, as well as biochemical compositions and the metabolic regulation mechanism in mixed sewage, were investigated. The results suggested that 18 was optimal N/P ratio, while the dry weight (1.0 g/L), chlorophyll-a (Chla) (3.63 mg/L), and lipid production (0.28 g/L) were all the highest comparing with other groups. In contrast, the protein production (0.37 g/L) was the least. The nature of the regulatory mechanisms inthe metabolic pathways of these biochemical compositions was revealed by proteomic results, and there were 62 different expression proteins (DEPs) taken part in fatty acid and lipid biosynthesis metabolism (FA), amino acid biosynthesis metabolism (AA), photosynthesis (PHO), carbon fixation in photosynthetic organisms (CFP), and central carbon metabolism (CCM).

Keywords: Chlorella pyrenoidosa; Mixed sewage; N/P ratio; Proteomics.

MeSH terms

Biomass
Chlorella* / metabolism
Lipids
Microalgae* / metabolism
Nitrogen / metabolism
Phosphorus / metabolism
Proteomics
Wastewater

Substances

Wastewater
Lipids
Nitrogen
Phosphorus