Phenotypic and genotypic characteristics of beta-lactamase dominant with CARBA, AmpC, and ESBL-producing bacteria in municipal wastewater influent in Helsinki, Finland

Ananda Tiwari; Jaana Paakkanen; Ahmad I Al-Mustapha; Juha Kirveskari; Rene S Hendriksen; Annamari Heikinheimo

doi:10.1016/j.jgar.2023.04.015

Phenotypic and genotypic characteristics of beta-lactamase dominant with CARBA, AmpC, and ESBL-producing bacteria in municipal wastewater influent in Helsinki, Finland

J Glob Antimicrob Resist. 2023 Jun:33:345-352. doi: 10.1016/j.jgar.2023.04.015. Epub 2023 May 9.

Authors

Ananda Tiwari¹, Jaana Paakkanen², Ahmad I Al-Mustapha¹, Juha Kirveskari², Rene S Hendriksen³, Annamari Heikinheimo⁴

Affiliations

¹ Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, Finland.
² HUSLAB, Helsinki, Finland.
³ Technical University of Denmark, National Food Institute (DTU Food), WHO Collaborating Centre for Antimicrobial Resistance in Foodborne Pathogens and Genomics, FAO Reference Laboratory for Antimicrobial Resistance (FAO RL), European Union Reference Laboratory for Antimicrobial Resistance (EURL-AMR). Kongens Lyngby, Denmark.
⁴ Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, Finland; Finnish Food Authority, Seinäjoki, Finland. Electronic address: annamari.heikinheimo@helsinki.fi.

PMID: 37169125
DOI: 10.1016/j.jgar.2023.04.015

Abstract

Objectives: Analysing samples of municipal wastewater influent (before treatment) can help to map the status of antibiotic-resistant bacteria (ARB) at the population level in sewershed communities and may also help in predicting the public health risks of ARB in surface water because of the outfall of wastewater. In this study, we investigated the bacterial isolates carrying beta-lactamase genes in wastewater and compared their genotypic and phenotypic characteristics.

Methods: A total of 399 bacterial isolates grown on CHROMagarESBL (n = 207) and CHROMagarKPC (n = 192) from composite wastewater influent samples (n = 7) from the Viikinmäki wastewater treatment plant (Helsinki) were subcultured, nucleic acid was extracted, and the prevalence of different beta-lactamase genes was screened with multiplex polymerase chain reaction (PCR). All PCR-positive isolates were identified with MALDI-TOF.

Results: A total of 32.6% of isolates (130 of 399) were PCR positive for at least one resistance gene, and 13% of these positive isolates out of 130 had at least three resistance genes. Among the 22 detected genes, bla_{GES group} was the most prevalent, at 25.8% (n = 198; many isolates carried multiple genes), followed by bla_MOX (13.1%) and bla_TEM (10.1%) as most frequently detected. Furthermore, out of 18 different bacterial species/genera detected as carrying beta-lactamase genes, A. hydrophila/caviae (28.5%), Enterobacter spp. (16.9%), and E. coli (14.6%) were the most prevalent. Enterobacter spp., Aeromonas spp., and K. cryocescens potentially carried AmpC genes, and E. coli carried ESBL genes.

Conclusion: We recorded a huge variety of beta-lactamases (bla_Amp_C, bla_ESBL, and bla_CARBA) genes in many potential pathogens that probably originated from both enteric and environmental sources.

Keywords: Aeromonas spp.; AmpC; ESBL; Enterobacter spp.; Enterobacteriaceae.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Angiotensin Receptor Antagonists
Angiotensin-Converting Enzyme Inhibitors
Enterobacter / genetics
Escherichia coli* / genetics
Finland
Humans
Multiplex Polymerase Chain Reaction
Wastewater
beta-Lactamases* / analysis
beta-Lactamases* / genetics

Substances

beta-Lactamases
Wastewater
Angiotensin Receptor Antagonists
Angiotensin-Converting Enzyme Inhibitors