Resveratrol alleviates lipopolysaccharide-induced liver injury by inducing SIRT1/P62-mediated mitophagy in gibel carp (Carassius gibelio)

Front Immunol. 2023 Apr 24:14:1177140. doi: 10.3389/fimmu.2023.1177140. eCollection 2023.

Abstract

Introduction: Resveratrol (RES) is a polyphenol organic compound with antioxidant and anti-inflammatory properties. This study aimed to determine whether and how RES can alleviate liver injury in lipopolysaccharide (LPS)-induced gibel carp.

Methods: Gibel carp were fed a diet with or without RES and were cultured for 8 weeks, followed by LPS injection.

Results and discussion: The results suggested that RES attenuated the resulting oxidative stress and inflammation by activating the Nrf2/Keap1 pathway and inhibiting the NF-κB pathway, as confirmed by changes in oxidative stress, inflammation-related gene expression, and antioxidant enzyme activity. Furthermore, RES cleared damaged mitochondria and enhanced mitochondrial biogenesis to mitigate reactive oxygen species (ROS) accumulation by upregulating the SIRT1/PGC-1α and PINK1/Parkin pathways and reducing p62 expression. Overall, RES alleviated LPS-induced oxidative stress and inflammation in gibel carp through mitochondria-related mechanisms.

Keywords: inflammation; lipopolysaccharide; mitophagy; oxidative stress; resveratrol.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / metabolism
  • Antioxidants / pharmacology
  • Carps* / metabolism
  • Chemical and Drug Induced Liver Injury, Chronic*
  • Cyprinidae* / metabolism
  • Inflammation / chemically induced
  • Inflammation / drug therapy
  • Kelch-Like ECH-Associated Protein 1 / metabolism
  • Lipopolysaccharides / toxicity
  • Mitophagy
  • NF-E2-Related Factor 2 / metabolism
  • Resveratrol / pharmacology
  • Sirtuin 1 / metabolism

Substances

  • Resveratrol
  • Antioxidants
  • Lipopolysaccharides
  • Kelch-Like ECH-Associated Protein 1
  • Sirtuin 1
  • NF-E2-Related Factor 2

Grants and funding

This research was financially supported by the National Natural Science Foundation of China (U19A2041; 32122089; 31972805; 32102811), the earmarked fund for CARS (CARS-45) and the National Key Research and Development Program of China (2018YFD0900605; 2019YFD0900200).