Biodegradation of clopidogrel bisulfate by Pseudomonas aeruginosa and Pseudomonas putida strains isolated from Algerian wastewater

Abstract

The clopidogrel bisulfate was degraded under aerobic conditions by two bacterial strains isolated from industrial effluents in El-Harrach, Algeria. The sequencing of their 16S rRNA revealed that these two strains are Pseudomonas aeruginosa and Pseudomonas putida. The experiments showed that this consortium could remove clopidogrel bisulfate at high concentrations (5-1500 mg·L^-1) within 96 h incubation period. The HPLC analysis recorded 75.23% degradation of clopidogrel bisulfate at an initial concentration of 100 mg·L^-1 after five days of incubation at pH 7.0 and a temperature of 30 °C. Also, a maximum degradation of 99.08% was carried out at a more basic pH (8.5). While only 41% was degraded at a temperature of 20 °C. Moreover, the presence of supplemental sources of carbon and nitrogen in the mixed culture media effectively improved the biodegradation of clopidogrel bisulfate by the stains. Finally, the morphology of the strains and the properties of the cell's surface were studied using a scanning electron microscope (SEM). This study reports, for the first time, the viability of the aerobic biodegradation of clopidogrel bisulfate in water in a wide range of concentrations.

Keywords: Biodegradation; Clopidogrel bisulfate; Industrial wastewater; Pseudomonas aeruginosa; Pseudomonas putida.