UPF1 regulates FOXO1 protein expression by promoting PBK transcription in non-small cell lung cancer

Peng Wang; Tianjiao Li; Lei Fang; Di Chen; Huan Qi; Chundong Gu

doi:10.1016/j.bbrc.2023.05.001

UPF1 regulates FOXO1 protein expression by promoting PBK transcription in non-small cell lung cancer

Biochem Biophys Res Commun. 2023 Jul 23:666:10-20. doi: 10.1016/j.bbrc.2023.05.001. Epub 2023 May 5.

Authors

Peng Wang¹, Tianjiao Li¹, Lei Fang¹, Di Chen², Huan Qi³, Chundong Gu⁴

Affiliations

¹ Department of Thoracic Surgery, Lung Cancer Diagnosis and Treatment Center of Dalian, The First Affiliated Hospital of Dalian Medical University, Dalian, 116011, China; Dalian Medical University, Dalian, 116011, China.
² CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.
³ CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China. Electronic address: huan.qi@dicp.ac.cn.
⁴ Department of Thoracic Surgery, Lung Cancer Diagnosis and Treatment Center of Dalian, The First Affiliated Hospital of Dalian Medical University, Dalian, 116011, China; Dalian Medical University, Dalian, 116011, China. Electronic address: guchundong@dmu.edu.cn.

PMID: 37167719
DOI: 10.1016/j.bbrc.2023.05.001

Abstract

Up-frameshift protein 1 (UPF1) is essential for nonsense-mediated messenger RNA decay (NMD). It is best known for its cytoprotective role in degrading aberrant and specific RNAs. UPF1 is dysregulated in multiple tumors, which correlates with poor prognosis and low overall survival.However,the role of UPF1 in lung cancer remains unclear.Current study shows that UPF1 could be a potential target for oncology therapies. The results also demonstrated the potential efficiency of UPF1 in regulating the proliferation and metastasis of lung cancer. Our findings suggest that those functions can be attributed to the inhibition of the stability of FOXO1 protein. In addition, PBK participates in the regulation of FOXO1 by UPF1.This result provides a new therapeutic strategy for lung cancer patients.

Keywords: Lung cancer; foxo1; pbk; upf1.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carcinoma, Non-Small-Cell Lung* / genetics
Forkhead Box Protein O1 / genetics
Forkhead Box Protein O1 / metabolism
Humans
Lung Neoplasms* / genetics
Nonsense Mediated mRNA Decay
RNA Helicases / genetics
RNA Helicases / metabolism
Trans-Activators / genetics
Trans-Activators / metabolism

Substances

Trans-Activators
Forkhead Box Protein O1
RNA Helicases
FOXO1 protein, human
UPF1 protein, human