Cerium oxide nanoparticles (nCeO2) exert minimal adverse effects on microbial communities in soils with and without biosolids amendment

Abstract

Increased use of nano-cerium oxide (nCeO₂) in an array of industrial applications has raised environmental concerns due to potential increased loadings to the soil environment. This research investigated the potential adverse effects of nCeO₂ (10-30 nm) on the soil microbial community in two exposure scenarios: direct application to soil, and indirect application to soil through chemical spiking of biosolids, followed by mixing into soil. Total Ce in test soils without, and with biosolids amendment, ranged from 44 to 770, and 73 to 664 mg Ce kg^-1 soil, respectively. In order to help distinguish whether observed effects were elicited by the solid-phase colloids or the activity of dissolved Ce, a soluble Ce salt (Ce (NO₃)₃) treatment was included in select assays. A suite of tests was used to investigate effects on critical processes: microbial growth (heterotrophic plate count), microbial activity (organic matter (OM) decomposition, enzyme activity and, nitrification) and diversity (structural and functional). Although results showed significant inhibition on microbial growth in soil without biosolids amendment at ≥ 156 mg Ce kg^-1 soil by week 5, these results were inconsistent and non-significant thereafter. In general, nCeO₂ showed no evidence of consistent adverse effects on OM decomposition, nitrification, soil enzyme activities and functional diversity. Leucine aminopeptidase showed significant (p< 0.05) stimulatory effects over time at ≥ 44 mg Ce kg^-1 in soils without biosolids, which was not observed in soils with biosolids amendment. The lack of inhibitory effects of nCeO₂ may be attributed to its low solubility; Ce in soil extracts (0.01 M CaCl₂) were all below detection (< 0.003 mg kg^-1) in the nCeO₂-spiked soils, but detectable in the Ce (NO₃)₃ samples. In contrast, soluble Ce at 359 mg Ce kg^-1 showed a significant reduction in OM decomposition and effects on microbial genomic diversity based on the 16S rDNA data in soils with and without biosolids amendment (359 and 690 mg Ce kg^-1). The nCeO₂ behaviour and effects information described herein are expected to help fulfill data gaps for the characterization of this priority nanomaterial.

Keywords: Cerium oxide; biosolids; nanoparticles; soil microbial community; toxicity.