Design, Assembly, and Function of DNA Origami Mechanisms

Peter E Beshay; Joshua A Johson; Jenny V Le; Carlos E Castro

doi:10.1007/978-1-0716-3028-0_2

Design, Assembly, and Function of DNA Origami Mechanisms

Methods Mol Biol. 2023:2639:21-49. doi: 10.1007/978-1-0716-3028-0_2.

Authors

Peter E Beshay¹, Joshua A Johson², Jenny V Le², Carlos E Castro^{3

4}

Affiliations

¹ Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH, USA.
² Biophysics Graduate Program, The Ohio State University, Columbus, OH, USA.
³ Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH, USA. castro.39@osu.edu.
⁴ Biophysics Graduate Program, The Ohio State University, Columbus, OH, USA. castro.39@osu.edu.

PMID: 37166709
DOI: 10.1007/978-1-0716-3028-0_2

Abstract

This chapter provides an overview of the common procedures used in making functional DNA origami devices. These procedures include the design, assembly, purification, and characterization of the DNA origami structures, with a focus on dynamic devices.

Keywords: Biomolecular nanotechnology; DNA nanotechnology; DNA origami; Dynamic nanodevices; Gel electrophoresis; Self-assembly; Transmission electron microscopy.

MeSH terms

DNA / chemistry
DNA / genetics
Nanostructures* / chemistry
Nanotechnology* / methods
Nucleic Acid Conformation

Substances

DNA