Hydroponic-based systems for plant growth allow control of the nutrients that plants take up through the provided nutrient solution. Different formulations of nutrient solutions enable the study of nutrient deficiencies in plants. Here we describe a procedure for setting up a simple semi-hydroponic system to grow maize seedlings. The system can be set up on a small or large scale, depending on the number of individuals studied. A modified nutrient solution is used for growing maize seedlings in iron-replete and iron-depleted conditions. This setup allows for studies of iron-deficiency responses in maize.
Keywords: Gene expression; Hydroponicsiron; Iron nutrition; Maize; Zea mays.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.