Scaling up human mesenchymal stem cell manufacturing using bioreactors for clinical uses

Marina Gazdic Jankovic; Miodrag Stojkovic; Sanja Bojic; Nemanja Jovicic; Marina Miletic Kovacevic; Zeljko Ivosevic; Aleksandar Juskovic; Vojin Kovacevic; Biljana Ljujic

doi:10.1016/j.retram.2023.103393

Scaling up human mesenchymal stem cell manufacturing using bioreactors for clinical uses

Curr Res Transl Med. 2023 Apr-Jun;71(2):103393. doi: 10.1016/j.retram.2023.103393. Epub 2023 Apr 28.

Authors

Marina Gazdic Jankovic¹, Miodrag Stojkovic², Sanja Bojic³, Nemanja Jovicic⁴, Marina Miletic Kovacevic⁴, Zeljko Ivosevic⁵, Aleksandar Juskovic⁶, Vojin Kovacevic⁷, Biljana Ljujic⁵

Affiliations

¹ University of Kragujevac, Serbia, Faculty of Medical Sciences, Department of Genetics, Serbia. Electronic address: marinagazdic87@gmail.com.
² SPEBO Medical Fertility Hospital, Leskovac, Serbia.
³ Newcastle University, School of Computing, Newcastle upon Tyne, UK.
⁴ University of Kragujevac, Serbia, Faculty of Medical Sciences, Department of Histology and Embryology, Serbia.
⁵ University of Kragujevac, Serbia, Faculty of Medical Sciences, Department of Genetics, Serbia.
⁶ Department of Orthopaedic Surgery, Clinical Centre of Montenegro, 81110 Podgorica, Montenegro.
⁷ University of Kragujevac, Serbia, Faculty of Medical Sciences, Department of Surgery, Serbia.

PMID: 37163885
DOI: 10.1016/j.retram.2023.103393

Abstract

Human mesenchymal stem cells (hMSCs) are multipotent cells and an attractive therapeutic agent in regenerative medicine and intensive clinical research. Despite the great potential, the limitation that needs to be overcome is the necessity of ex vivo expansion because of insufficient number of hMSCs presented within adult organs and the high doses required for a transplantation. As a result, numerous research studies aim to provide novel expansion methods in order to achieve appropriate numbers of cells with preserved therapeutic quality. Bioreactor-based cell expansion provide high-level production of hMSCs in accordance with good manufacturing practice (GMP) and quality standards. This review summarizes current knowledge about the hMSCs manufacturing platforms with a main focus to the application of bioreactors for large-scale production of GMP-grade hMSCs.

Keywords: Bioreactor; Manufacturing; Mesenchymal stem cells.

Publication types

Review

MeSH terms

Adult
Bioreactors
Cell Culture Techniques* / methods
Cell Proliferation
Cells, Cultured
Humans
Mesenchymal Stem Cells*