Laundry detergents and surfactants-induced eosinophilic airway inflammation by increasing IL-33 expression and activating ILC2s

Kyoko Saito; Keisuke Orimo; Terufumi Kubo; Masato Tamari; Ayako Yamada; Kenichiro Motomura; Hiroki Sugiyama; Ryo Matsuoka; Naoko Nagano; Yuka Hayashi; Ken Arae; Mariko Hara; Masashi Ikutani; Tatsuki Fukuie; Katsuko Sudo; Akio Matsuda; Yukihiro Ohya; Shigeharu Fujieda; Hirohisa Saito; Susumu Nakae; Kenji Matsumoto; Cezmi A Akdis; Hideaki Morita

doi:10.1111/all.15762

Laundry detergents and surfactants-induced eosinophilic airway inflammation by increasing IL-33 expression and activating ILC2s

Allergy. 2023 Jul;78(7):1878-1892. doi: 10.1111/all.15762. Epub 2023 May 23.

Authors

Kyoko Saito^{1

2}, Keisuke Orimo^{1

3}, Terufumi Kubo⁴, Masato Tamari^{1

5}, Ayako Yamada¹, Kenichiro Motomura¹, Hiroki Sugiyama^{1

6}, Ryo Matsuoka^{1

5}, Naoko Nagano^{1

7}, Yuka Hayashi^{1

8

9}, Ken Arae^{1

10}, Mariko Hara¹, Masashi Ikutani¹¹, Tatsuki Fukuie¹², Katsuko Sudo¹³, Akio Matsuda¹, Yukihiro Ohya¹², Shigeharu Fujieda², Hirohisa Saito¹, Susumu Nakae^{11

14}, Kenji Matsumoto¹, Cezmi A Akdis^{15

16}, Hideaki Morita^{1

12}

Affiliations

¹ Department of Allergy and Clinical Immunology, National Research Institute for Child Health and Development, Tokyo, Japan.
² Department of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medical Sciences, University of Fukui, Fukui, Japan.
³ Department of Respiratory Medicine, Tokyo Women's Medical University, Tokyo, Japan.
⁴ Department of Pathology, Sapporo Medical University School of Medicine, Hokkaido, Japan.
⁵ Department of Pediatrics, Jikei University School of Medicine, Tokyo, Japan.
⁶ Department of Pediatrics, Graduate School of Medicine, Yokohama City University, Yokohama, Japan.
⁷ Department of Respiratory Medicine, The University of Tokyo, Tokyo, Japan.
⁸ Department of Pediatrics, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
⁹ Department of Developmental Pediatrics, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
¹⁰ Department of Immunology, Faculty of Health Sciences, Kyorin University, Tokyo, Japan.
¹¹ Graduate School of Integrated Science for Life, Hiroshima University, Hiroshima, Japan.
¹² Allergy Center, National Center for Child Health and Development, Tokyo, Japan.
¹³ Pre-clinical Research Center, Tokyo Medical University, Tokyo, Japan.
¹⁴ Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency, Saitama, Japan.
¹⁵ Swiss Institute of Allergy and Asthma Research (SIAF), University of Zurich, Davos, Switzerland.
¹⁶ Christine Kühne - Center for Allergy Research and Education (CK-CARE), Davos, Switzerland.

PMID: 37163231
DOI: 10.1111/all.15762

Abstract

Introduction: Epidemiological studies demonstrated that cleaning work and frequent use of cleaning products are risk factors for asthma. Laundry detergents have been reported to have epithelial barrier-opening effects. However, whether laundry detergents directly induce airway inflammation and its mechanisms in vivo remain to be elucidated.

Methods: Two commercial laundry detergents and two commonly used surfactants for cleaning and cosmetics (sodium lauryl sulfate and sodium dodecyl benzene sulfonate) were intranasally administered to mice. Lungs were analyzed using flow cytometry, histology, ELISA, and quantitative PCR. Human bronchial epithelial cells were stimulated with laundry detergents and analyzed using quantitative PCR and western blotting. Involvement of oxidative stress was assessed using an antioxidant. Dust samples from homes were analyzed to determine their detergent content by measuring their critical micelle concentration (CMC).

Results: The administered laundry detergents and surfactants-induced eosinophilic airway inflammation accompanied by increased IL-33 expression and activation of group 2 innate lymphoid cells (ILC2s). Detergent-induced eosinophilic airway inflammation was significantly attenuated in Rag2^-/- Il2rg^-/- , Il33^-/- mice, and also in wild-type mice treated with NAC. Detergent-induced IL-33 expression in airways was attenuated by NAC treatment, both in vivo and in vitro. CMCs were found in all of the tested dust extracts, and they differed significantly among the homes.

Conclusion: The laundry detergents and surfactants-induced eosinophilic airway inflammation in vivo through epithelial cell and ILC2 activation. They induced IL-33 expression in airway epithelial cells through oxidative stress. Furthermore, detergent residues were present in house dust and are presumably inhaled into the airway in daily life.

Keywords: bronchial asthma; environment; group 2 innate lymphoid cells (ILC2); hygiene hypothesis; oxidative stress.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Detergents* / adverse effects
Dust
Humans
Immunity, Innate*
Inflammation
Interleukin-33 / pharmacology
Lymphocytes
Mice
Surface-Active Agents / adverse effects

Substances

Detergents
Surface-Active Agents
Interleukin-33
Dust

Grants and funding

SNSF_/Swiss National Science Foundation/Switzerland