Spontaneous mutations in hlyD and tuf genes result in resistance of Dickeya solani IPO 2222 to phage ϕD5 but cause decreased bacterial fitness and virulence in planta

Daryna Sokolova; Anna Smolarska; Przemysław Bartnik; Lukasz Rabalski; Maciej Kosinski; Magdalena Narajczyk; Dorota M Krzyżanowska; Magdalena Rajewska; Inez Mruk; Paulina Czaplewska; Sylwia Jafra; Robert Czajkowski

doi:10.1038/s41598-023-34803-7

Spontaneous mutations in hlyD and tuf genes result in resistance of Dickeya solani IPO 2222 to phage ϕD5 but cause decreased bacterial fitness and virulence in planta

Sci Rep. 2023 May 9;13(1):7534. doi: 10.1038/s41598-023-34803-7.

Authors

Affiliations

¹ Laboratory of Biologically Active Compounds, Intercollegiate Faculty of Biotechnology UG and MUG, University of Gdansk, A. Abrahama 58, 80-307, Gdansk, Poland.
² Department of Biophysics and Radiobiology, Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, 148 Academika Zabolotnoho St., Kyiv, 03143, Ukraine.
³ Department of Cancer Biology, Institute of Biology, Warsaw, University of Life Sciences (SGGW), J. Ciszewskiego 8, 02-786, Warsaw, Poland.
⁴ Laboratory of Recombinant Vaccines, Intercollegiate Faculty of Biotechnology UG and MUG, University of Gdansk, A. Abrahama 58, 80-307, Gdansk, Poland.
⁵ Laboratory of Electron Microscopy, Faculty of Biology, University of Gdansk, Wita Stwosza 59, 80-308, Gdansk, Poland.
⁶ Laboratory of Plant Microbiology, Intercollegiate Faculty of Biotechnology UG and MUG, University of Gdansk, A. Abrahama, 58, 80-307, Gdansk, Poland.
⁷ Laboratory of Mass Spectrometry-Core Facility Laboratories, Intercollegiate Faculty of Biotechnology UG and MUG, University of Gdansk, Antoniego Abrahama 58, 80-307, Gdansk, Poland.
⁸ Laboratory of Biologically Active Compounds, Intercollegiate Faculty of Biotechnology UG and MUG, University of Gdansk, A. Abrahama 58, 80-307, Gdansk, Poland. robert.czajkowski@ug.edu.pl.

Abstract

Lytic bacteriophages able to infect and kill Dickeya spp. can be readily isolated from virtually all Dickeya spp. containing environments, yet little is known about the selective pressure those viruses exert on their hosts. Two spontaneous D. solani IPO 2222 mutants (0.8% of all obtained mutants), DsR34 and DsR207, resistant to infection caused by lytic phage vB_Dsol_D5 (ΦD5) were identified in this study that expressed a reduced ability to macerate potato tuber tissues compared to the wild-type, phage-susceptible D. solani IPO 2222 strain. Genome sequencing revealed that genes encoding: secretion protein HlyD (in mutant DsR34) and elongation factor Tu (EF-Tu) (in mutant DsR207) were altered in these strains. These mutations impacted the DsR34 and DsR207 proteomes. Features essential for the ecological success of these mutants in a plant environment, including their ability to use various carbon and nitrogen sources, production of plant cell wall degrading enzymes, ability to form biofilms, siderophore production, swimming and swarming motility and virulence in planta were assessed. Compared to the wild-type strain, D. solani IPO 2222, mutants DsR34 and DsR207 had a reduced ability to macerate chicory leaves and to colonize and cause symptoms in growing potato plants.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteriophages*
Enterobacteriaceae
Mutation
Swimming
Virulence / genetics

Supplementary concepts

Dickeya solani