Mosquito larvae are aquatic and go through four development stages (larval instars L1-L4) before pupation. Species vary in the duration of larval development, and a variety of external factors affect the development rate (e.g., water temperature, food type, and larval density), which are discussed more thoroughly elsewhere. Here, we detail how to rear Anopheles larvae. This protocol describes appropriate distribution of larvae into rearing pans, feeding of larvae, cleaning of pans, and care until pupation.
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