Haplotype-resolved genome assembly of Coriaria nepalensis a non-legume nitrogen-fixing shrub

Sci Data. 2023 May 9;10(1):259. doi: 10.1038/s41597-023-02171-6.

Abstract

Coriaria nepalensis Wall. (Coriariaceae) is a nitrogen-fixing shrub which forms root nodules with the actinomycete Frankia. Oils and extracts of C. nepalensis have been reported to be bacteriostatic and insecticidal, and C. nepalensis bark provides a valuable tannin resource. Here, by combining PacBio HiFi sequencing and Hi-C scaffolding techniques, we generated a haplotype-resolved chromosome-scale genome assembly for C. nepalensis. This genome assembly is approximately 620 Mb in size with a contig N50 of 11 Mb, with 99.9% of the total assembled sequences anchored to 40 pseudochromosomes. We predicted 60,862 protein-coding genes of which 99.5% were annotated from databases. We further identified 939 tRNAs, 7,297 rRNAs, and 982 ncRNAs. The chromosome-scale genome of C. nepalensis is expected to be a significant resource for understanding the genetic basis of root nodulation with Frankia, toxicity, and tannin biosynthesis.

Publication types

  • Dataset

MeSH terms

  • Chromosomes, Plant
  • Genome, Plant*
  • Haplotypes
  • Magnoliopsida* / genetics
  • Molecular Sequence Annotation
  • Phylogeny