An aptamer-functionalized photonic crystal sensor for ultrasensitive and label-free detection of aflatoxin B1

Mingdi Jia; Licheng Yu; Xiaoxuan Li; Yijun Li; Xiwen He; Langxing Chen; Yukui Zhang

doi:10.1016/j.talanta.2023.124638

An aptamer-functionalized photonic crystal sensor for ultrasensitive and label-free detection of aflatoxin B1

Talanta. 2023 Aug 1:260:124638. doi: 10.1016/j.talanta.2023.124638. Epub 2023 May 3.

Authors

Mingdi Jia¹, Licheng Yu², Xiaoxuan Li³, Yijun Li⁴, Xiwen He⁵, Langxing Chen⁶, Yukui Zhang⁷

Affiliations

¹ College of Chemistry, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Nankai University, Tianjin, 300071, China. Electronic address: 2120200724@mail.nankai.edu.cn.
² College of Chemistry, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Nankai University, Tianjin, 300071, China. Electronic address: 2120180870@mail.nankai.edu.cn.
³ College of Chemistry, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Nankai University, Tianjin, 300071, China. Electronic address: 2120180761@mail.nankai.edu.cn.
⁴ College of Chemistry, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Nankai University, Tianjin, 300071, China; National Demonstration Center for Experimental Chemistry Education (Nankai University), Tianjin, 300071, China. Electronic address: yijunli@nankai.edu.cn.
⁵ College of Chemistry, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Nankai University, Tianjin, 300071, China. Electronic address: xiwenhe@nankai.edu.cn.
⁶ College of Chemistry, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Nankai University, Tianjin, 300071, China. Electronic address: lxchen@nankai.edu.cn.
⁷ College of Chemistry, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Nankai University, Tianjin, 300071, China; Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116011, China. Electronic address: ykzhang@dicp.ac.cn.

PMID: 37156207
DOI: 10.1016/j.talanta.2023.124638

Abstract

As a novel optical responsive material, photonic crystal is a promising sensing material in the recognition and detection of small molecules. Herein, a label-free composite sensor for aflatoxin B1 (AFB1) based on aptamer-functionalized photonic crystal arrays was successfully developed. Three-dimensional photonic crystals (3D PhCs) with a controllable number of layers were produced by a layer-by-layer (LBL) approach, and the introduction of gold nanoparticles (AuNPs) facilitated the immobilization procedure of recognition element aptamers, thus creating the AFB1 sensing detection system (AFB1-Apt 3D PhCs). The sensing system AFB1-Apt 3D PhCs exhibited a good linearity in the wide range of 1 pg mL^-1-100 ng mL^-1 AFB1 with a limit of detection (LOD) of 0.28 pg mL^-1. Furthermore AFB1-Apt 3D PhC was successfully applied in the determination of AFB1 in the millet and beer samples with good recovery. The sensing system performed ultrasensitive and label-free detection to the target, which could be further applied in the fields of food safety, clinical diagnosis or environmental monitoring, establishing an efficient and rapid universal detection platform.

Keywords: Aflatoxin B1; Aptamer; Food samples; Gold nanoparticles; Three-dimensional photonic crystal.

MeSH terms

Aflatoxin B1 / analysis
Aptamers, Nucleotide* / chemistry
Biosensing Techniques* / methods
Gold / chemistry
Limit of Detection
Metal Nanoparticles* / chemistry

Substances

Aflatoxin B1
Gold
Aptamers, Nucleotide