Calcium-binding properties, stability, and osteogenic ability of phosphorylated soy peptide-calcium chelate

Xiao Kong; Ziqun Xiao; Yuhang Chen; MengDi Du; Zihui Zhang; Zhenhua Wang; Bo Xu; Yongqiang Cheng; Tianying Yu; Jing Gan

doi:10.3389/fnut.2023.1129548

Calcium-binding properties, stability, and osteogenic ability of phosphorylated soy peptide-calcium chelate

Front Nutr. 2023 Apr 21:10:1129548. doi: 10.3389/fnut.2023.1129548. eCollection 2023.

Authors

Xiao Kong¹, Ziqun Xiao^{1

2

3}, Yuhang Chen^{1

2}, MengDi Du¹, Zihui Zhang¹, Zhenhua Wang¹, Bo Xu¹, Yongqiang Cheng², Tianying Yu¹, Jing Gan¹

Affiliations

¹ Center for Mitochondria and Healthy Aging, College of Life Science, Yantai University, Yantai, Shandong, China.
² Beijing Key Laboratory of Functional Food from Plant Resources, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.
³ School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China.

Abstract

Introduction: Bioactive peptides based on foodstuffs are of particular interest as carriers for calcium delivery due to their safety and high activity. The phosphorylated peptide has been shown to enhance calcium absorption and bone formation.

Method: A novel complex of peptide phosphorylation modification derived from soybean protein was introduced, and the mechanism, stability, and osteogenic differentiation bioactivity of the peptide with or without calcium were studied.

Result: The calcium-binding capacity of phosphorylated soy peptide (SPP) reached 50.24 ± 0.20 mg/g. The result of computer stimulation and vibration spectrum showed that SPP could chelate with calcium by the phosphoric acid group, carboxyl oxygen of C-terminal Glu, Asp, and Arg, and phosphoric acid group of Ser on the SPP at a stoichiometric ratio of 1:1, resulting in the formation of the complex of ligand and peptide. Thermal stability showed that chelation enhanced peptide stability compared with SPP alone. Additionally, in vitro results showed that SPP-Ca could facilitate osteogenic proliferation and differentiation ability.

Discussion: SPP may function as a promising alternative to current therapeutic agents for bone loss.

Keywords: calcium supplement; characterization; osteogenic differentiation; peptide-calcium chelate; phosphorylation; thermal stability.

Grants and funding

This study was supported by the National Natural Science Foundation of China (31871450) and the Natural Science Foundation of Shandong (ZR2022MC217). This study was also partly supported by the Center for Mitochondria and Healthy Aging, College of Life Sciences, Yantai University.