Bioreactor systems for micropropagation of plants: present scenario and future prospects

Hosakatte Niranjana Murthy; Kadanthottu Sebastian Joseph; Kee Yoeup Paek; So Young Park

doi:10.3389/fpls.2023.1159588

Bioreactor systems for micropropagation of plants: present scenario and future prospects

Front Plant Sci. 2023 Apr 19:14:1159588. doi: 10.3389/fpls.2023.1159588. eCollection 2023.

Authors

Hosakatte Niranjana Murthy^{1

2}, Kadanthottu Sebastian Joseph³, Kee Yoeup Paek², So Young Park²

Affiliations

¹ Department of Botany, Karnatak University, Dharwad, India.
² Department of Horticultural Science, Chungbuk National University, Cheongju, Republic of Korea.
³ Department of Life Sciences, CHRIST (Deemed to be University), Bengaluru, India.

Abstract

Plant micropropagation has been adapted in the fields of agriculture, horticulture, forestry, and other related fields for large-scale production of elite plants. The use of liquid media and adoption of bioreactors have escalated the production of healthy plants. Several liquid-phase, gas-phase, temporary immersion, and other modified bioreactors have been used for plant propagation. The design, principle, operational mode, merits, and demerits of various bioreactors used for the regeneration of propagules, such as bulblets, cormlets, rhizomes, microtubers, shoots (subsequent rooting), and somatic embryos, are discussed here. In addition, various parameters that affect plant regeneration are discussed with suitable examples.

Keywords: Bioreactor; embryogenesis; immersion culture; in vitro propagation; liquid culture; organogenesis; somatic embryo; temporary immersion culture.

Publication types

Review

Grants and funding

This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT) (No. NRF-2020R1A2C2102401) and Technology Innovation Program funded by the Ministry of Trade, Industry & Energy (MOTIE, Korea) (Grant No. P0018148).