Necroptosis-related lncRNAs: establishment of a gene module and distinction between the cold and hot tumors in glioma

Kangxi Cao; Fengbo Su; Xuchun Shan; Xingyu Jiang; Zhaohui Ni; Yan Chen

doi:10.3389/fonc.2023.1087117

Necroptosis-related lncRNAs: establishment of a gene module and distinction between the cold and hot tumors in glioma

Front Oncol. 2023 Apr 21:13:1087117. doi: 10.3389/fonc.2023.1087117. eCollection 2023.

Authors

Kangxi Cao¹, Fengbo Su¹, Xuchun Shan², Xingyu Jiang², Zhaohui Ni², Yan Chen¹

Affiliations

¹ Department of Neurosurgery, The Second Hospital of Jilin University, Changchun, China.
² Department of Pathogenobiology, The Key Laboratory of Zoonosis, Chinese Ministry of Education, College of Basic Medical Sciences, Jilin University, Changchun, China.

Abstract

Background: Gliomas are the most common primary tumors of the central nervous system and portend a poor prognosis. The efficacy of emerging and promising immunotherapies varies significantly among individuals. Distinction and transformation of cold and hot tumors may improve the antitumor efficacy of immunotherapy.

Methods and results: In this study, we constructed a necroptosis-related lncRNA module based on public databases. The association of this module with survival was assessed using the Cox regression, Kaplan-Meier survival analysis, and nomogram, external validation was also conducted in another public database. Furthermore, we performed gene set enrichment analysis (GSEA), immune checkpoint and tumor microenvironment analysis, and in vitro qRT-PCR validation. Finally, we clustered all samples into 2 clusters based on the expression of model lncRNAs and identified cluster 1 as cold tumors with fewer infiltrating T cells.

Conclusions: Identifying cold and hot tumors by necroptosis-related lncRNAs can help available immunotherapeutic strategies to achieve efficacy in the precise treatment of individuals. Prior treatment failure can be overcome by targeting necroptosis-related lncRNAs.

Keywords: cold and hot tumors; glioma; lncRNA; necroptosis; prognosis.

Grants and funding

This work was supported by the Jilin Province Science and Technology Department [grant numbers 20200201510JC, 20210402021GH]; and the Education Department of Jilin Province [grant number JJKH20211147KJ].