Detection of orthodontically induced inflammatory root resorption-associated biomarkers from the gingival crevicular fluid by proteomics analysis: a randomized-controlled clinical trial

Harraa S Mohammed-Salih; Hadeel Adel Al-Lami; Hayder F Saloom; Ali Abbas Abdulkareem; Jameel R Al-Obaidi; Nurhanani Razali

doi:10.1007/s13205-023-03572-5

Detection of orthodontically induced inflammatory root resorption-associated biomarkers from the gingival crevicular fluid by proteomics analysis: a randomized-controlled clinical trial

3 Biotech. 2023 May;13(5):157. doi: 10.1007/s13205-023-03572-5. Epub 2023 May 2.

Authors

Harraa S Mohammed-Salih¹, Hadeel Adel Al-Lami¹, Hayder F Saloom¹, Ali Abbas Abdulkareem², Jameel R Al-Obaidi³, Nurhanani Razali⁴

Affiliations

¹ Department of Orthodontics, College of Dentistry, University of Baghdad, Baghdad, 10011 Iraq.
² Department of Periodontics, College of Dentistry, University of Baghdad, Baghdad, 10011 Iraq.
³ Department of Biology, Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris, 35900 Tanjong Malim, Perak Malaysia.
⁴ Membranology Unit, Okinawa Institute of Science and Technology Graduate University, 1919-1, Tancha, Onna-son, Kunigami-kun, Okinawa, 904-0495 Japan.

Abstract

Orthodontically induced inflammatory root resorption (OIIRR) is an undesirable complication of orthodontic treatment (OT) with an ambiguous aetiologic mechanism. This study aimed to identify OIIRR-associated biomarkers in the gingival crevicular fluid (GCF) using proteomic analysis. In this randomized clinical trial, the upper first premolars (UFP) were exposed either to light or heavy force. The GCF was collected at 1 h, 1 day, 7 days, 14 days, 21 days, and 28 days following force application. After extraction of UFP, roots were imaged and resorption premolar, was used to deliver either light forcecraters were measured. Proteomic analysis of GCF was performed using 2D gel electrophoresis with MALDI-TOF/TOF MS/MS. Results were further analyzed by bioinformatics analyses showing the biological functions and predicted pathways. The predicted canonical pathways showed that the expression of immunoglobulin kappa (IGKC), neutrophil gelatinase-associated lipocalin (NGAL), neurolysin mitochondrial (NEUL), keratin, type II cytoskeletal 1 (K2C1), S100-A9, and the extracellular calcium-sensing receptor (CASR) were significantly associated with a range of biological and inflammatory processes. In conclusion, up-regulation of S100A9, CASR, and K2C1 suggested a response to force-related inflammation, chemotactic activities, osteoclastogenesis, and epithelial cell breakdown. Meanwhile, the up-regulation of IGKC, NGAL, and K2C1 indicated a response to the inflammatory process, innate immunity activation, and epithelial cell breakdown.

Supplementary information: The online version contains supplementary material available at 10.1007/s13205-023-03572-5.

Keywords: Gingival crevicular fluid (GCF); Proteomic; Root resorption.

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