Association of IL-17A promoter region SNP-rs2275913 with urinary bladder cancer

Maryam Anwar; Muhammad Ejaz; Muhammad Ijaz; Gulzar Ahmad; Humaira Ayub; Syeda Hafiza Benish Ali

Association of IL-17A promoter region SNP-rs2275913 with urinary bladder cancer

Int J Health Sci (Qassim). 2023 May-Jun;17(3):33-38.

Authors

Maryam Anwar^{1

2}, Muhammad Ejaz³, Muhammad Ijaz¹, Gulzar Ahmad¹, Humaira Ayub^{2

4}, Syeda Hafiza Benish Ali⁵

Affiliations

¹ Institute of Integrative Biosciences, CECOS University, Peshawar, Pakistan.
² COMSATS University, Islamabad, Pakistan.
³ Ayub Teaching Hospital, Abbottabad, Pakistan.
⁴ Department of Biomedical Sciences, Pak-Austria Fachhochschule Institute of Applied Sciences and Technology, Pakistan.
⁵ Department of Biosciences, COMSATS University, Islamabad, Pakistan.

PMID: 37151747
PMCID: PMC10155249

Abstract

Objective: Urinary bladder cancer (UBC) is the fourth most common cancer among men and tenth most common cancer in women. This study investigated an association of interleukins -17A promoter region single nucleotide polymorphism (SNP)-rs2275913 with UBC in Pakistani population.

Methods: Population-based study was designed with 127 UBC patients and 100 healthy individuals. Only UBC Patients were included and other diseases hepatitis or any other malignancy/cancer were excluded from the study. Polymerase chain reaction Restriction fragment length polymorphism technique was used to genotype the rs2275913 SNP in patients and control. Linear regression analysis was performed on the genotype data and allelic frequency data. Online statistical tool was used to calculate ratio of odds.

Results: Linear regression analysis showed that there was no association between rs2275913 SNP and UBC patients in the dominant model (OR = 0.815, CI = 0.415-1.6), recessive model (OR = 0.389, CI = 0.014-5.565), codominant model (OR = 0.376, CI=0.013-5.420) and (OR = 0.855, CI = 0.427-1.713). Moreover, among the UBC samples, low-grade non-muscle invasive UBC samples dominant model (OR = 0.722, CI = 0.316-1.637), recessive model (OR = 0.000, CI = 0.000-5.864), codominant model (OR = 0.864, CI = 0.030-12.668), and (OR = 0.788, CI = 0.341-1.806) did also not show any association. When same analysis was performed for high-grade muscle invasive UBC, dominant (OR = 0.936, CI = 0.403-2.155), recessive model (OR = 0.875, CI = 0.031-12.696), and codominant model (OR = 0.864, CI = 0.030-12.668,), and (OR = 0.942, CI = 0.394-2.232) did not show any association.

Conclusion: Results revealed that rs2275913 did not show any associated with the high risk of UBC in Pakistani population. Some limitations of the studies are firstly, the samples size and other are detailed information on UBC and role of inflammation.

Keywords: Urinary bladder cancer; interleukins-17A; linear regression; polymerase chain reaction-restriction fragment length polymorphism; single nucleotide polymorphism.