Immune Cell Profiling of Atopic Dermatitis Patients Before and After Treatment with Halometasone Cream Wet-Wrap Therapy by Single-Cell Sequencing

Xiao-Guang Gu; Xin Yu; Bo-Yang Zhou; Ming Li; Wei Xu; Yan Li; Lin-Feng Li

doi:10.4103/ijd.ijd_801_22

Immune Cell Profiling of Atopic Dermatitis Patients Before and After Treatment with Halometasone Cream Wet-Wrap Therapy by Single-Cell Sequencing

Indian J Dermatol. 2023 Jan-Feb;68(1):8-14. doi: 10.4103/ijd.ijd_801_22.

Authors

Xiao-Guang Gu¹, Xin Yu¹, Bo-Yang Zhou², Ming Li², Wei Xu², Yan Li², Lin-Feng Li²

Affiliations

¹ Department of Dermatology and Venerology, Aviation General Hospital, Xicheng, Beijing, China.
² Department of Dermatology, Beijing Friendship Hospital, Capital Medical University, Xicheng, Beijing, China.

Abstract

Objectives: Peripheral blood immune cell profiling of atopic dermatitis patients before and after treatment by single-cell RNA sequencing technique has not been reported. To study the immune Cell Profiling of Atopic Dermatitis Patients Before and After Treatment with Halometasone Cream Wet-Wrap Therapy.

Methods: We used single cell sequencing to detect the proportion change and gene expression change of immune cells in 2 patients before and after treatment, and then used real-time PCR to confirm the mRNA level of differential genes.

Results: In this study, scRNA-seq in two patients with severe AD before and after halometasone cream wet-wrap therapy showed that in the mild severity of AD after treatment, Th2 cells were significantly decreased (41.2% vs 13.4%), Th1 and Th17 cells were increased (23.3% vs 43.7%, 2.3% vs 4.8% respectively). The proportion of Th22 cells did not change much (1.3% vs 1.9%). Tregs were significantly increased also (1.5% vs 5.0%). In the regulatory T cells, the expression of IL-27, PD-1, CD103, CTLA-4, ZNF-66, IL-β, CD7 gene was specifically increased after treatment, and CD39, P21, TOX2, CD151, CD79A, S100A12, TRAP1 gene was specifically decreased after treatment. In the TH2 cells, the expression of CD27, CD68, EZH1, RAD1, EGFR, CCR10, BCL11A, KLF4 gene was specifically increased after treatment and CCL26, CD180, IL-31, CCL22, LEF1, OX40 gene was specifically decreased after treatment.

Conclusions: These genes may be new target for further study.

Keywords: CD39; TH2 cells; TOX2; Tregss; single-cell RNA sequencing technique.