Characterization of a novel sRNA contributing to biofilm formation in Salmonella enterica serovar Typhimurium

Sayema Naaz; Najmuj Sakib; Dominika Houserova; Rani Badve; Aline Crucello; Glen M Borchert

doi:10.17912/micropub.biology.000796

Characterization of a novel sRNA contributing to biofilm formation in Salmonella enterica serovar Typhimurium

MicroPubl Biol. 2023 Apr 20:2023:10.17912/micropub.biology.000796. doi: 10.17912/micropub.biology.000796. eCollection 2023.

Authors

Sayema Naaz^#¹, Najmuj Sakib^#¹, Dominika Houserova¹, Rani Badve¹, Aline Crucello¹, Glen M Borchert^#¹

Affiliation

¹ Department of Pharmacology, College of Medicine, University of South Alabama, Mobile, AL.

^# Contributed equally.

Abstract

Small RNAs (sRNAs) are short noncoding RNAs of ~50-200 nucleotides believed to primarily function in regulating crucial activities in bacteria during periods of cellular stress. This study examined the relevance of specific sRNAs on biofilm formation in nutrient starved Salmonella enterica serovar Typhimurium. Eight unique sRNAs were selected for deletion primarily based on their genomic location and/or putative targets. Quantitative and qualitative analyses confirm one of these, sRNA1186573, is required for efficient biofilm formation in S. enterica further highlighting the significance of sRNAs during Salmonella stress response.

Grants and funding

Funding was provided in part by NSF grant NSF2219900 (GMB) and NSF CAREER grant 1350064 (with co-funding provided by the NSF EPSCoR program) (GMB) both awarded by the Division of Molecular and Cellular Biosciences. Graduate funding was also provided in part by Alabama Commission on Higher Education ALEPSCoR grant 180435 (DH). The project used an instrument funded, in part, by the National Science Foundation MRI Grant No. CNS-1726069. Research reported in this publication was also supported by the National Center for Advancing Translational Research of the National Institutes of Health under award number UL1TR001417 (GMB).