Comparative analysis of intercellular lipid organization and composition between psoriatic and healthy stratum corneum

Tomonobu Uchino; Daichi Kamiya; Hiroaki Yagi; Hiyori Fujino-Shimaya; Ichiro Hatta; Shun Fujimori; Yasunori Miyazaki; Yukako Kirishita; Yuko Sano; Hajime Mizuno; Kenichiro Todoroki; Yoshiyuki Kagawa

doi:10.1016/j.chemphyslip.2023.105305

Comparative analysis of intercellular lipid organization and composition between psoriatic and healthy stratum corneum

Chem Phys Lipids. 2023 Aug:254:105305. doi: 10.1016/j.chemphyslip.2023.105305. Epub 2023 May 6.

Authors

Affiliations

¹ Department of Clinical Pharmaceutics, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan; Laboratory of Clinical Pharmacokinetics, the Medical Frontier Center, Shizuoka General Hospital, 4-27-1 Kita Ando, Aoi-ku, Shizuoka 420-8527, Japan. Electronic address: uchinot@u-shizuoka-ken.ac.jp.
² Department of Clinical Pharmaceutics, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan.
³ Department of Dermatology, Shizuoka General Hospital, 4-27-1 Kita Ando, Aoi-ku, Shizuoka 420-8527, Japan.
⁴ Department of Research, Nagoya Industrial Science Research Institute, 1-13 Yotsuyadori, Chikusa-ku, Nagoya 464-0819, Japan.
⁵ Department of Clinical Pharmaceutics, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan; Laboratory of Clinical Pharmacokinetics, the Medical Frontier Center, Shizuoka General Hospital, 4-27-1 Kita Ando, Aoi-ku, Shizuoka 420-8527, Japan.
⁶ Laboratory of Analytical and Bio‑Analytical Chemistry, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga‑ku, Shizuoka 422-8526, Japan.

PMID: 37150244
DOI: 10.1016/j.chemphyslip.2023.105305

Abstract

The lipid composition and organization of the stratum corneum (SC) in patients with psoriasis and healthy subjects were compared using X-ray diffraction, Fourier-transform infrared spectroscopy (FT-IR), and ultraperformance liquid chromatography, combined with time-of-flight mass spectrometry (UPLC-TOFMS). In healthy SC (HSC), SC lipids formed two lamellar phases (long and short periodicity phases). Hexagonal and orthorhombic hydrocarbon-chain packing were observed in the lateral lipid organization at 30 °C via X-ray diffraction. In HSC, the lamellar phases and the hydrocarbon-chain packing organizations changed with elevated temperatures and finally disappeared. In these behaviors, the high-temperature hexagonal hydrocarbon-chain packing organization, which appeared above the orthorhombic hydrocarbon-chain packing organization, transformed to the liquid phase at about 90 °C in HSC. In psoriatic SC (PSC), hexagonal hydrocarbon-chain packing organization disappeared at about 65 °C with elevated temperatures. No high-temperature hexagonal hydrocarbon-chain packing organization were observed in PSC during heating process. Disorder of the hydrocarbon-chain packing of SC lipids was observed in PSC via FT-IR. In UPLC-TOFMS, free fatty acid (FFA) and ceramide (CER) compositions differed between patients with PSC and HSC. Specifically, the levels of ultra-long chain fatty acids containing CER and phytosphingosine-containing CER were decreased, while those of sphingosine and dihydrosphingosine-containing CER and unsaturated FFA were increased in PSC. Furthermore, FFA and CER carbon chain lengths decreased in patients with PSC. These results suggest that the alteration of SC lipid composition and the reduction of carbon chain lengths in PSC lowered the structural transformation temperature, thereby reducing barrier function.

Keywords: Lamellar organization; Lateral lipid organization; Lipid chain length; Psoriasis; Stratum corneum; Synchrotron X-ray diffraction.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Ceramides / chemistry
Epidermis* / chemistry
Fatty Acids / analysis
Fatty Acids, Nonesterified* / analysis
Fatty Acids, Nonesterified* / chemistry
Humans
Skin / chemistry
Spectroscopy, Fourier Transform Infrared
X-Ray Diffraction

Substances

Fatty Acids, Nonesterified
Fatty Acids
Ceramides