Rapid and inexpensive voltammetric detection of ochratoxin A in wheat matrices

Yaser Arteshi; Dhésmon Lima; Sheryl A Tittlemier; Sabine Kuss

doi:10.1016/j.bioelechem.2023.108451

Rapid and inexpensive voltammetric detection of ochratoxin A in wheat matrices

Bioelectrochemistry. 2023 Aug:152:108451. doi: 10.1016/j.bioelechem.2023.108451. Epub 2023 Apr 28.

Authors

Yaser Arteshi¹, Dhésmon Lima², Sheryl A Tittlemier³, Sabine Kuss⁴

Affiliations

¹ Department of Chemistry, University of Manitoba, 144, Dysart Road, R3T 2N2 Winnipeg, MB, Canada. Electronic address: arteshiy@myumanitoba.ca.
² Department of Chemistry, University of Manitoba, 144, Dysart Road, R3T 2N2 Winnipeg, MB, Canada. Electronic address: dhesmon.lima@umanitoba.ca.
³ Canadian Grain Commission, 303, Main Street, R3C 3G8, Winnipeg, MB, Canada. Electronic address: sheryl.tittlemier@grainscanada.gc.ca.
⁴ Department of Chemistry, University of Manitoba, 144, Dysart Road, R3T 2N2 Winnipeg, MB, Canada. Electronic address: sabine.kuss@umanitoba.ca.

PMID: 37150089
DOI: 10.1016/j.bioelechem.2023.108451

Abstract

Produced as toxic metabolites by fungi, mycotoxins, such as ochratoxin A (OTA), contaminate grain and animal feed and cause great economic losses. Herein, we report the fabrication of an electrochemical sensor consisting of an inexpensive and label-free carbon black-graphite paste electrode (CB-G-CPE), which was fully optimized to detect OTA in durum wheat matrices using differential pulse voltammetry (DPV). The effect of carbon paste composition, electrolyte pH and DPV parameters were studied to determine the optimum conditions for the electroanalytical determination of OTA. Full factorial and central composite experimental designs (FFD and CCD) were used to optimize DPV parameters, namely pulse width, pulse height, step height and step time. The developed electrochemical sensor successfully detected OTA with detection and quantification limits equal to 57.2 nM (0.023 µg mL^-1) and 190.6 nM (0.077 µg mL^-1), respectively. The accuracy and precision of the presented CB-G-CPE was used to successfully quantify OTA in real wheat matrices. This study presents an inexpensive and user-friendly method with potential applications in grain quality control.

Keywords: Carbon-black; Carbon-paste-electrode; Differential pulse voltammetry; Electrochemical sensor; Grain samples; Ochratoxin A.

MeSH terms

Animals
Carbon / chemistry
Electrochemical Techniques / methods
Electrodes
Graphite* / chemistry
Triticum*

Substances

ochratoxin A
Carbon
Graphite