Bioinspired Polymer Assemblies of Plant Cell Walls for Measuring Protein-Carbohydrate Interactions by FRAP

Berangère Lebas; Gabriel Paës

doi:10.1007/978-1-0716-3151-5_11

Bioinspired Polymer Assemblies of Plant Cell Walls for Measuring Protein-Carbohydrate Interactions by FRAP

Methods Mol Biol. 2023:2657:151-162. doi: 10.1007/978-1-0716-3151-5_11.

Authors

Berangère Lebas¹, Gabriel Paës²

Affiliations

¹ Fractionation of AgroResources and Environment (FARE) laboratory, INRAE, Université de Reims Champagne-Ardenne, Reims, France.
² Fractionation of AgroResources and Environment (FARE) laboratory, INRAE, Université de Reims Champagne-Ardenne, Reims, France. gabriel.paes@inrae.fr.

PMID: 37149529
DOI: 10.1007/978-1-0716-3151-5_11

Abstract

The interactions of proteins involved in plant cell wall hydrolysis, such as enzymes and CBMs, significantly determine their role and efficiency. In order to go beyond the characterization of interactions with simple ligands, bioinspired assemblies combined with the measurement of diffusion and interaction by FRAP offer a relevant alternative for highlighting the importance of different parameters related to the protein affinity and to the polymer type and organization in the assembly.

Keywords: Bioinspired; CBM; Diffusion; Enzyme; FRAP; Interaction; Ligand; Protein.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbohydrates
Cell Membrane
Cell Wall* / metabolism
Polymers* / metabolism

Substances

Polymers
Carbohydrates