Hemocytes in blue mussel Mytilus edulis adopt different energy supply modes to cope with different BDE-47 exposures

Yongshun Jiang; Sai Cao; Bin Zhou; Qiyue Cao; Mengxue Xu; Tianli Sun; Xinyu Zhao; Zhongyuan Zhou; You Wang

doi:10.1016/j.scitotenv.2023.163766

Hemocytes in blue mussel Mytilus edulis adopt different energy supply modes to cope with different BDE-47 exposures

Sci Total Environ. 2023 Aug 10:885:163766. doi: 10.1016/j.scitotenv.2023.163766. Epub 2023 May 3.

Authors

Yongshun Jiang¹, Sai Cao², Bin Zhou³, Qiyue Cao⁴, Mengxue Xu⁵, Tianli Sun⁶, Xinyu Zhao⁷, Zhongyuan Zhou⁸, You Wang⁹

Affiliations

¹ School of Marine Science and Engineering, Qingdao Agricultural University, No. 17 Wenhai Road, Qingdao, China; Laboratory for Marine Ecology and Environmental Science, Laoshan Laboratory, No. 1 Wenhai Road, Qingdao, China. Electronic address: jys@qau.edu.cn.
² Department of Marine Ecology, College of Marine Life Sciences, Ocean University of China, No. 5 Yushan Road, Qingdao, China; Laboratory for Marine Ecology and Environmental Science, Laoshan Laboratory, No. 1 Wenhai Road, Qingdao, China. Electronic address: caosai@stu.ouc.edu.cn.
³ Department of Marine Ecology, College of Marine Life Sciences, Ocean University of China, No. 5 Yushan Road, Qingdao, China; Laboratory for Marine Ecology and Environmental Science, Laoshan Laboratory, No. 1 Wenhai Road, Qingdao, China. Electronic address: zhoubin@ouc.edu.cn.
⁴ Department of Marine Ecology, College of Marine Life Sciences, Ocean University of China, No. 5 Yushan Road, Qingdao, China.
⁵ Department of Marine Ecology, College of Marine Life Sciences, Ocean University of China, No. 5 Yushan Road, Qingdao, China; Marine Science Research Institute of Shandong Province, Qingdao 266104, China.
⁶ National Marine Hazard Mitigation Service, No. 6, Qiwangfen North Road, Beijing, China. Electronic address: tlsun@nmhms.org.cn.
⁷ Laboratory for Marine Ecology and Environmental Science, Laoshan Laboratory, No. 1 Wenhai Road, Qingdao, China.
⁸ Department of Marine Ecology, College of Marine Life Sciences, Ocean University of China, No. 5 Yushan Road, Qingdao, China; Laboratory for Marine Ecology and Environmental Science, Laoshan Laboratory, No. 1 Wenhai Road, Qingdao, China. Electronic address: zhouzhongyuan@ouc.edu.cn.
⁹ Department of Marine Ecology, College of Marine Life Sciences, Ocean University of China, No. 5 Yushan Road, Qingdao, China; Laboratory for Marine Ecology and Environmental Science, Laoshan Laboratory, No. 1 Wenhai Road, Qingdao, China. Electronic address: wangyou@ouc.edu.cn.

PMID: 37146804
DOI: 10.1016/j.scitotenv.2023.163766

Abstract

The energetic response of blue mussel Mytilus edulis when coping with tetrabromodiphenyl ether (BDE-47) exposure was evaluated from the perspective of alterations in energy supply mode, and the possible regulating mechanism was discussed based on a 21-day bioassay. The results showed that the energy supply mode changed with concentration: 0.1 μg/L BDE-47 decreased the activity of isocitrate dehydrogenase (IDH), succinate dehydrogenase (SDH), malate dehydrogenase and oxidative phosphorylation, suggesting inhibition of the tricarboxylic (TCA) acid cycle and aerobic respiration. The coincident increase in phosphofructokinase and the decrease in lactate dehydrogenase (LDH) indicated that glycolysis and anaerobic respiration were increased. When exposed to 1.0 μg/L BDE-47, M. edulis mainly utilized aerobic respiration, but lowered glucose metabolism as indicated by the decrease in glutamine and l-leucine was suggested to be involved in this process, which was differed from that in the control. The reoccurrence of IDH and SDH inhibition as well as LDH elevation indicated attenuation of aerobic and anaerobic respiration when the concentration increased to 10 μg/L, but severe protein damage was evidenced based on the elevation of amino acids and glutamine. Under the 0.1 μg/L BDE-47, activation of the AMPK-Hif-1a signaling pathway promoted the expression of glut1, which was the potential mechanism for the improvement of anaerobic respiration, and further activated glycolysis and anaerobic respiration. This study shows that the energy supply mode experienced a conversion from aerobic respiration under normal conditions to anaerobic mode in the low BDE-47 treatment and back to aerobic respiration with increasing BDE-47 concentrations, which may represent a potential mechanism for mussel physiological responses when faced with different levels of BDE-47 stress.

Keywords: 2,2′,4,4′-Tetrabromodiphenyl ether (BDE-47); AMPK-Hif-1α signal pathway; Blue mussel; Energy supply mode; Hemocytes.

MeSH terms

Animals
Glutamine
Halogenated Diphenyl Ethers / toxicity
Hemocytes
Mytilus edulis*
Mytilus*

Substances

2,2',4,4'-tetrabromodiphenyl ether
Glutamine
Halogenated Diphenyl Ethers