Construction of PEGylated chlorin e6@CuS-Pt theranostic nanoplatforms for nanozymes-enhanced photodynamic-photothermal therapy

Yangyi Sheng; Qian Ren; Cheng Tao; Mei Wen; Pu Qu; Nuo Yu; Maoquan Li; Zhigang Chen; Xiaoyun Xie

doi:10.1016/j.jcis.2023.04.092

Construction of PEGylated chlorin e6@CuS-Pt theranostic nanoplatforms for nanozymes-enhanced photodynamic-photothermal therapy

J Colloid Interface Sci. 2023 Sep:645:122-132. doi: 10.1016/j.jcis.2023.04.092. Epub 2023 Apr 24.

Authors

Yangyi Sheng¹, Qian Ren¹, Cheng Tao², Mei Wen², Pu Qu², Nuo Yu², Maoquan Li³, Zhigang Chen⁴, Xiaoyun Xie⁵

Affiliations

¹ Department of Interventional and Vascular Surgery, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China; State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, China.
² State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, China.
³ Department of Interventional and Vascular Surgery, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China.
⁴ Department of Interventional and Vascular Surgery, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China; State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, China. Electronic address: zgchen@dhu.edu.cn.
⁵ Department of Interventional and Vascular Surgery, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China. Electronic address: xiaoyunxietj@126.com.

PMID: 37146376
DOI: 10.1016/j.jcis.2023.04.092

Abstract

Multifunctional nanoagents with photodynamic therapy (PDT) and photothermal therapy (PTT) functions have shown great promise for cancer treatment, while the design and synthesis of efficient nanoagents remain a challenge. To realize nanozyme-enhanced PDT-PTT combined therapy, herein we have synthesized the Ce6@CuS-Pt/PEG nanoplatforms as a model of efficient nanoagents. Hollow CuS nanospheres with an average diameter of ∼ 200 nm are first synthesized through vulcanization using Cu₂O as the precursor. Subsequently, CuS nanospheres are surface-decorated with Pt nanoparticles (NPs) as nanozyme via an in-situ reduction route, followed by modifying the DSPE-PEG5000 and loading the photosensitizer Chlorin e6 (Ce6). The obtained Ce6@CuS-Pt/PEG NPs exhibit high photothermal conversion efficiency (43.08%), good singlet oxygen (¹O₂) generation ability, and good physiological stability. In addition, Ce6@CuS-Pt/PEG NPs show good catalytic performance due to the presence of Pt nanozyme, which can effectively convert H₂O₂ to O₂ and significantly enhance the production of cytotoxic ¹O₂. When Ce6@CuS-Pt/PEG NPs dispersion is injected into mice, the tumors can be wholly suppressed owing to nanozyme-enhanced PDT-PTT combined therapy, providing better therapeutic effects compared to single-mode phototherapy. Thus, the present Ce6@CuS-Pt/PEG NPs can act as an efficient multifunctional nanoplatform for tumor therapy.

Keywords: Ce6@CuS-Pt/PEG nanoplatforms; Nanozymes; Photodynamic therapy; Photothermal therapy; Tumor.

MeSH terms

Animals
Cell Line, Tumor
Hydrogen Peroxide
Mice
Nanoparticles* / therapeutic use
Nanospheres*
Photochemotherapy*
Photosensitizing Agents
Photothermal Therapy
Polyethylene Glycols
Porphyrins* / pharmacology
Precision Medicine

Substances

phytochlorin
Hydrogen Peroxide
Photosensitizing Agents
Polyethylene Glycols
Porphyrins