Synergy among extracellular adsorption, bio-precipitation and transmembrane transport of Penicillium oxalicum SL2 enhanced Pb stabilization

Jianhao Tong; Binhui Ye; Xiaohan Jiang; Hanxin Wu; Qiao Xu; Yating Luo; Jingli Pang; Fei Jia; Jiyan Shi

doi:10.1016/j.jhazmat.2023.131537

Synergy among extracellular adsorption, bio-precipitation and transmembrane transport of Penicillium oxalicum SL2 enhanced Pb stabilization

J Hazard Mater. 2023 Jul 15:454:131537. doi: 10.1016/j.jhazmat.2023.131537. Epub 2023 Apr 28.

Authors

Jianhao Tong¹, Binhui Ye², Xiaohan Jiang¹, Hanxin Wu¹, Qiao Xu³, Yating Luo¹, Jingli Pang¹, Fei Jia⁴, Jiyan Shi⁵

Affiliations

¹ Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China.
² Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China; Zhejiang Guyu Ecological Environment Technology Co., Ltd, Hangzhou 310000, China.
³ Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China; Key Laboratory of Urban Environment and Health, Ningbo Urban Environment Observation and Research Station, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China.
⁴ Zhejiang Jiuhe Geological and Ecological Environment Planning and Design Company, Huzhou 313002, China.
⁵ Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address: shijiyan@zju.edu.cn.

PMID: 37146333
DOI: 10.1016/j.jhazmat.2023.131537

Abstract

As a potential bioremediation strain for Pb contamination, Penicillium oxalicum SL2 sometimes has secondary activation of Pb, so it is crucial to clarify its effect on Pb morphology and its intracellular response to Pb stress. We investigated the effect of P. oxalicum SL2 in medium on Pb²⁺ and Pb availability in eight minerals, and revealed the prioritization of Pb products. (i)Pb was stabilized within 30 days as Pb₃(PO₄)₂ or Pb₅(PO₄)₃Cl with sufficient phosphorus (P); (ii) under P deficiency but sulfur (S) sufficient, Pb was stabilized mainly in the form of PbSO₄; (iii) under conditions of P and S deficiency, Pb was stabilized mainly in the form of PbC₂O₂. With the help of proteomic and metabolomics analysis, a total of 578 different proteins and 194 different metabolites were found to be matched in 52 pathways. Among them, the activation of chitin synthesis, oxalate production, sulfur metabolism and transporters improved the Pb tolerance of P. oxalicum SL2, and promoted the synergistic effect of extracellular adsorption, bio-precipitation and transmembrane transport on Pb stabilization. Our results fill the gap in the intracellular response of P. oxalicum SL2 to Pb and provide new insights into the development of bioremediation agent and technology for Pb contamination.

Keywords: Bioremediation; Metabolomics; Pb; Penicillium oxalicum SL2; Proteomics.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adsorption
Biodegradation, Environmental*
Lead* / metabolism
Penicillium*
Proteomics
Sulfur / metabolism

Substances

Lead
Sulfur

Supplementary concepts

Penicillium oxalicum