Complete genome sequence of a novel closterovirus isolated from Dregea volubilis

Shangyun Li; Rex Frimpong Anane; Zeli Chen; Chunfang Duan; Zhe Wang; Like Gao; Daihong Yu; Bifan Chu; Zefen Yang; Guosong Wen; Mingfu Zhao

doi:10.1007/s00705-023-05775-w

Complete genome sequence of a novel closterovirus isolated from Dregea volubilis

Arch Virol. 2023 May 5;168(5):154. doi: 10.1007/s00705-023-05775-w.

Authors

Shangyun Li^#^{1

2

3}, Rex Frimpong Anane^#⁴, Zeli Chen^{1

2

3}, Chunfang Duan⁵, Zhe Wang^{1

3}, Like Gao^{1

3}, Daihong Yu⁶, Bifan Chu^{1

3}, Zefen Yang^{1

2

3}, Guosong Wen^{1

2

3}, Mingfu Zhao^{7

8}

Affiliations

¹ Key Laboratory for Agricultural Biodiversity for Pest Management of China, Ministry of Education, Yunnan Agricultural University, No. 95, Jinhei Road, Panlong District, Kunming, Yunnan, 650201, China.
² College of Agronomy and Biotechnology, Yunnan Agricultural University, No. 95, Jinhei Road, Panlong District, Kunming, Yunnan, 650201, China.
³ Research and Development Center for Health Product, College of Agronomy and Biotechnology, Yunnan Agricultural University, No. 95, Jinhei Road, Kunming, Yunnan, 650201, China.
⁴ Monash Biomedicine Discovery Institute, Department of Biochemistry and Molecular Biology, Monash University, 23 Innovation Walk, Clayton, VIC, 3800, Australia.
⁵ Tropical and Subtropical Cash Crops Research Institute, Yunnan Academy of Agricultural Sciences, Baoshan, 678000, China.
⁶ Plant Protection and Quarantine Station of Yuanjiang County, Yuxi, Yunnan, 653300, China.
⁷ Key Laboratory for Agricultural Biodiversity for Pest Management of China, Ministry of Education, Yunnan Agricultural University, No. 95, Jinhei Road, Panlong District, Kunming, Yunnan, 650201, China. zhaomingfu@163.com.
⁸ Research and Development Center for Health Product, College of Agronomy and Biotechnology, Yunnan Agricultural University, No. 95, Jinhei Road, Kunming, Yunnan, 650201, China. zhaomingfu@163.com.

^# Contributed equally.

PMID: 37145182
DOI: 10.1007/s00705-023-05775-w

Abstract

The complete genome sequence of a putative novel closterovirus, tentatively named "Dregea volubilis closterovirus 1" (DvCV1, GenBank accession no. MZ779122), infecting Dregea volubilis in China was determined using high-throughput sequencing (HTS). The complete genome sequence of DvCV1 consists of 16,165 nucleotides (nt) and contains nine ORFs. The genome structure of DvCV1 is typical of members of the genus Closterovirus. Complete genome sequence analysis showed that DvCV1 shares 41.4-48.4% nucleotide sequence identity with other known closteroviruses. The putative RNA-dependent RNA polymerase (RdRp), heat shock protein 70-like protein (HSP70h), and coat protein (CP) of DvCV1 share 46.80-62.65%, 31.06-51.80%, and 28.34-37.37% amino acid sequence identity, respectively, with the RdRp, HSP70h and CP of other closteroviruses. Phylogenetic analysis based on HSP70h aa sequences placed DvCV1 alongside other members of the genus Closterovirus in the family Closteroviridae. These results suggest that DvCV1 is a new member of the genus Closterovirus. This is the first report of a closterovirus infecting D. volubilis.

Keywords: Closterovirus; Dregea volubilis; HTS.

MeSH terms

Closteroviridae* / genetics
Closterovirus* / genetics
Genome, Viral
Open Reading Frames
Phylogeny
Plant Diseases
RNA, Viral / genetics

Substances

RNA, Viral