The phase range extension and accuracy improvement in Fresnel biprism-based digital holography microscopy

Hasti Nourzadeh; Maryam Lotfi; Humberto Cabrera; Maria Liz Crespo; Masoomeh Dashtdar

doi:10.1063/5.0145654

The phase range extension and accuracy improvement in Fresnel biprism-based digital holography microscopy

Rev Sci Instrum. 2023 May 1;94(5):053703. doi: 10.1063/5.0145654.

Authors

Hasti Nourzadeh¹, Maryam Lotfi¹, Humberto Cabrera², Maria Liz Crespo³, Masoomeh Dashtdar^{1

2}

Affiliations

¹ Department of Physics, Shahid Beheshti University, Evin, Tehran 19839-69411, Iran.
² Optics Lab, STI Unit, The Abdus Salam International Centre for Theoretical Physics, Strada Costiera 11, Trieste 34151, Italy.
³ Multidisciplinary Laboratory (MLAB), STI Unit, The Abdus Salam International Centre for Theoretical Physics, Strada Costiera 11, Trieste 34151, Italy.

PMID: 37144944
DOI: 10.1063/5.0145654

Abstract

We report a highly stable and affordable dual-wavelength digital holographic microscopy system based on common-path geometry. A Fresnel biprism is used to create an off-axis geometry, and two diode laser sources with different wavelengths λ1 = 532 nm and λ2 = 650 nm generate the dual-wavelength compound hologram. In order to extend the measurement range, the phase distribution is obtained using a synthetic wavelength Λ1 = 2930.5 nm. Furthermore, to improve the system's temporal stability and reduce speckle noise, a shorter wavelength (Λ2 = 292.5 nm) is used. The feasibility of the proposed configuration is validated by the experimental results obtained with Molybdenum trioxide, Paramecium, and red blood cell specimens.