Methods and Protocols for Using Extracellular Vesicles as Delivery Vehicles in Neuronal Research

Manjusha Vaidya; Nasif Sayeed; Caroline Hobson; Sandeep Sreerama; Jonhoi Smith; Riya Shah; Kiminobu Sugaya

doi:10.1007/978-1-0716-3203-1_13

Methods and Protocols for Using Extracellular Vesicles as Delivery Vehicles in Neuronal Research

Methods Mol Biol. 2023:2668:159-189. doi: 10.1007/978-1-0716-3203-1_13.

Authors

Manjusha Vaidya¹, Nasif Sayeed¹, Caroline Hobson¹, Sandeep Sreerama¹, Jonhoi Smith¹, Riya Shah¹, Kiminobu Sugaya²

Affiliations

¹ Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, FL, USA.
² Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, FL, USA. ksugaya@ucf.edu.

PMID: 37140797
DOI: 10.1007/978-1-0716-3203-1_13

Abstract

Extracellular vesicles (EVs) transport nucleic acids, proteins, and lipid molecules for intercellular communication. The biomolecular cargo from EVs can modify the recipient cell genetically, physiologically, and pathologically. This innate ability of EVs can be harnessed to deliver the cargo of interest to a specific organ or a cell type. Importantly, due to their ability to cross the blood-brain barrier (BBB), the EVs can be used as delivery vehicles to transport therapeutic drugs and other macromolecules to inaccessible organs such as the brain. Therefore, the current chapter includes laboratory techniques and protocols focusing on the customization of EVs for neuronal research.

Keywords: Cargo delivery; Exosomes; Extracellular vesicles; Localization.

MeSH terms

Biological Transport
Blood-Brain Barrier / metabolism
Brain / metabolism
Extracellular Vesicles* / metabolism
Nucleic Acids* / metabolism

Substances

Nucleic Acids