A novel algicidal properties of fermentation products from Pseudomonas sp. Ps3 strain on the toxic red tide dinoflagellate species

Luwei Zheng; Hong Lin; Barathan Balaji-Prasath; Yuping Su; Ying Wang; Yi Zheng; Guanglang Yu

doi:10.3389/fmicb.2023.1146325

A novel algicidal properties of fermentation products from Pseudomonas sp. Ps3 strain on the toxic red tide dinoflagellate species

Front Microbiol. 2023 Apr 17:14:1146325. doi: 10.3389/fmicb.2023.1146325. eCollection 2023.

Authors

Luwei Zheng¹, Hong Lin¹, Barathan Balaji-Prasath^{1

2

3}, Yuping Su^{1

2

3}, Ying Wang¹, Yi Zheng⁴, Guanglang Yu¹

Affiliations

¹ College of Environmental and Resource Science, College of Carbon Neutral Modern Industry, Fujian Normal University, Fuzhou, China.
² Fujian Key Laboratory of Pollution Control and Resource Recycling, Fujian Normal University, Fuzhou, China.
³ Fujian Province Research Centre for River and Lake Health Assessment, Fujian Normal University, Fuzhou, China.
⁴ Fujian Key Laboratory of Special Marine Bio-resources Sustainable Utilization, Fujian Normal University, Fuzhou, China.

Abstract

The viability of both China's offshore fishing operations and the global marine fishing industry is threatened by the occurrence of red tides caused by Gymnodinium catenatum and Karenia mikimotoi. Effective control of these dinoflagellate-mediated red tides has become a pressing issue that requires immediate attention. In this study, High-efficiency marine alginolytic bacteria were isolated and underwent molecular biological identification to confirm their algicidal properties. Based on a combination of morphological, physiological, biochemical, and sequencing results, Strain Ps3 was identified as belonging to the species Pseudomonas sp. We examine the effects of algicidal bacteria on the red tide species G. catenatum and K. mikimotoi within an indoor experimental setting. Then gas chromatography- mass spectrometry (GC-MS) was used to analyze the structure of the algolytic active substances. This investigation demonstrated that with exposure to the algae-lysis experiment, the Ps3 strain has the best algae-lysis effect, with G. catenatum and K. mikimotoi reaching 83.0 and 78.3%. Our results from the sterile fermentation broth experiment showed that the inhibitory effect on the two red tide algae was positively correlated with the concentration of the treatment. At a treatment concentration of 2.0% (v/v), the 48 h lysis rates of G. catenatum and K. mikimotoi due to exposure to the Ps3 bacterial fermentation broth were 95.2 and 86.7%, respectively. The results of this study suggest that the algaecide may be a rapid and effective method to control dinoflagellate blooms, as evidenced by the observed changes in cellular morphology in all cases. In the ethyl acetate phase of Ps3 fermentation broth, the cyclic (leucine-leucine) dipeptide was the most abundant. The findings of this study contribute to our understanding of red tide prevention and control and provide a theoretical foundation for further research in this field.

Keywords: Pseudomonas; active substances; algicidal bacteria; dinoflagellate; red tide.