Effect of silk fibroin scaffold loaded with 17-β estradiol on the proliferation and differentiation of BMSCs

Juan Bai; Haotian Li; Lu Wang; Yue Shi; Xiaomin Su; Changzhen Xu; Qiaoling Guo; Jing Feng; Xilin Sun; Yitong Cheng; Jie Kang; Jiayu Wen; Feng Wu

doi:10.1016/j.reth.2023.03.002

Effect of silk fibroin scaffold loaded with 17-β estradiol on the proliferation and differentiation of BMSCs

Regen Ther. 2023 Apr 21:23:76-83. doi: 10.1016/j.reth.2023.03.002. eCollection 2023 Jun.

Authors

Juan Bai^{1

2}, Haotian Li³, Lu Wang^{1

2}, Yue Shi², Xiaomin Su², Changzhen Xu², Qiaoling Guo², Jing Feng², Xilin Sun², Yitong Cheng², Jie Kang², Jiayu Wen², Feng Wu²

Affiliations

¹ Department of Prosthodontics, Shanxi Medical University School and Hospital of Stomatology, Taiyuan, 030001, China.
² Shanxi Province Key Laboratory of Oral Diseases Prevention and New Materials, Taiyuan, 030001, China.
³ First Clinical Medical College of Shanxi Medical University, Taiyuan, 030001, China.

Abstract

In this study, different concentrations of 17-β estradiol silk fibroin (SF)porous scaffolds (SFPS) were prepared using freeze-drying technique, with a hope for optimal concentration and apply it locally to the bone defect area. In this study, the porous scaffold morphology structure was characterized by SEM, FTIR and universal capacity testing machines, and the in vitro cytocompatibility and biological activity of scaffold materials were studied by cell adhesion, viability and proliferation experiments. The results showed that SFPS boasts better physicochemical properties, while 17-β estradiol SF scaffolds with low concentrations of 10^-10 mol/L and 10^-12 mol/L had more growth and proliferation of SF scaffolds with higher concentrations, and 10^-10 mol/L was the optimal concentration of 17-β estradiol SFPS, which was more conducive to cell adhesion and proliferation. On the other hand, after osteogenesis induction of BMSCs inoculated on 17-β estradiol SFPS at different concentrations, it was found that the expression of alkaline phosphatase in BMSCs on different concentrations of 17-β estradiol porous scaffolds was not large. No conflict of interest exits in the submission of this manuscript.

Keywords: 17-β estradiol; BMSCs; Silk fibroin; Silk fibroin porous scaffolds.