Lipidomics insight on differences between human MFGM and dietary-derived lipids

Shaolei Wang; Cristabelle De Souza; Mythili Ramachandran; Ya Luo; Yixin Zhang; Huaxi Yi; Zhao Ma; Lanwei Zhang; Kai Lin

doi:10.1016/j.foodchem.2023.136236

Lipidomics insight on differences between human MFGM and dietary-derived lipids

Food Chem. 2023 Oct 1:422:136236. doi: 10.1016/j.foodchem.2023.136236. Epub 2023 Apr 27.

Authors

Shaolei Wang¹, Cristabelle De Souza², Mythili Ramachandran³, Ya Luo⁴, Yixin Zhang⁴, Huaxi Yi⁴, Zhao Ma⁵, Lanwei Zhang⁶, Kai Lin⁷

Affiliations

¹ College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China; National Center of Technology Innovation for Dairy, Hohhot 010000, China.
² Department of Stem Cell Research and Regenerative Medicine, School of Medicine, Stanford University, Stanford, CA 94305, USA.
³ Department of Biochemistry and Molecular Medicine, UC Davis Comprehensive Cancer Center, University of California Davis, Sacramento, CA 95817, USA.
⁴ College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
⁵ Department of Medicinal Chemistry, Key Laboratory of Chemical Biology (MOE), School of Pharmacy, Cheeloo College of Medicine, Shandong University, Jinan 250012 Shandong, China.
⁶ College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China; National Center of Technology Innovation for Dairy, Hohhot 010000, China. Electronic address: zhanglanwei@ouc.edu.cn.
⁷ College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China; National Center of Technology Innovation for Dairy, Hohhot 010000, China. Electronic address: linkai@ouc.edu.cn.

PMID: 37130453
DOI: 10.1016/j.foodchem.2023.136236

Abstract

Milk fat globule membrane (MFGM) contains lipids, which are essential for promoting infant brain development and improving cognition. In this study, the lipid differences between human MFGM and four dietary lipid sources (cow MFGM, soybean, krill, and yolk) were compared using the UHPLC-Q-Exactive MS-based lipidomics techniques. A total of 45 lipid classes and 5048 lipid species were detected. The analysis of phospholipid classes revealed that the lipid composition of human MFGM and cow MFGM was more similar than the other dietary-derived lipids. Additionally, the human MFGM lipid species were compared with cow MFGM, soybean, krill, and yolk, and 401, 416, 494, and 444 significantly different lipids were identified, respectively. Through lipid metabolic pathway analysis, differential lipids were mainly involved in the glycerophospholipid metabolic pathway. Overall, these results will provide a rationale for the future addition of lipids to infant formula to more closely approximate human MFGM lipid profiles.

Keywords: Lipidomics; Milk fat globule membrane; Non-milk lipids; Phospholipid; UHPLC-Q-Exactive MS.

MeSH terms

Animals
Brain / metabolism
Cattle
Dietary Fats
Female
Glycolipids*
Humans
Infant
Infant Formula
Lipid Droplets / metabolism
Lipidomics*
Phospholipids

Substances

Glycolipids
Dietary Fats
Phospholipids