Exploring substrate interaction in respiratory alternative complex III from Rhodothermus marinus

Biochim Biophys Acta Bioenerg. 2023 Aug 1;1864(3):148983. doi: 10.1016/j.bbabio.2023.148983. Epub 2023 Apr 29.

Abstract

Rhodothermus marinus is a thermohalophilic organism that has optimized its microaerobic metabolism at 65 °C. We have been exploring its respiratory chain and observed the existence of a quinone:cytochrome c oxidoreductase complex, named Alternative Complex III, structurally different from the bc1 complex. In the present work, we took profit from nanodiscs and liposomes technology to investigate ACIII activity in membrane-mimicking systems. In addition, we studied the interaction of ACIII with menaquinone, its potential electron acceptors (HiPIP and cytochrome c) and the caa3 oxygen reductase.

Keywords: Alternative complex III (ACIII); Cytochrome bc1; High Potential Iron Sulfur Protein (HiPIP); Menaquinone; Respiratory chain; Thermophile.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cytochromes c*
  • Electron Transport
  • Electron Transport Complex III*
  • Oxidoreductases

Substances

  • Electron Transport Complex III
  • Cytochromes c
  • Oxidoreductases

Supplementary concepts

  • Rhodothermus marinus