Tuber indicum and T. lijiangense colonization differentially regulates plant physiological responses and mycorrhizosphere bacterial community of Castanopsis rockii seedlings

Lanlan Huang; Yongmei Li; Jing Yuan; Shanping Wan; Carlos Colinas; Xinhua He; Xiaofei Shi; Yanliang Wang; Fuqiang Yu

doi:10.3389/fpls.2023.1134446

Tuber indicum and T. lijiangense colonization differentially regulates plant physiological responses and mycorrhizosphere bacterial community of Castanopsis rockii seedlings

Front Plant Sci. 2023 Apr 12:14:1134446. doi: 10.3389/fpls.2023.1134446. eCollection 2023.

Authors

Lanlan Huang^{1

2}, Yongmei Li¹, Jing Yuan², Shanping Wan¹, Carlos Colinas³, Xinhua He^{2

4

5}, Xiaofei Shi^{2

6}, Yanliang Wang², Fuqiang Yu²

Affiliations

¹ College of Resources and Environment, Yunnan Agricultural University, Kunming, China.
² The Germplasm Bank of Wild Species, Yunnan Key Laboratory for Fungal Diversity and Green Development, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan, China.
³ Department of Crop and Forest Science, University of Lleida, Lleida, Spain.
⁴ Centre of Excellence for Soil Biology, College of Resources and Environment, and Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, School of Life Sciences, Southwest University, Chongqing, China.
⁵ School of Biological Sciences, University of Western Australia, Perth, WA, Australia.
⁶ Guizhou Kangqunyuan Biotechnology Co., LTD, Liupanshui, Guizhou, China.

Abstract

Black truffles and white truffles are widely studied around the world, but their effects on plant growth and physiological responses, and on the mycorrhizosphere bacterial community of the host plant remain unclear. Here, mycorrhizal colonization of Castanopsis rockii by Tuber indicum (Chinese black truffle) and T. lijiangense (Chinese white truffle), respectively, was induced in a greenhouse study, and their effects on host growth, physiological responses and mycorrhizosphere bacterial communities were compared. The results show that colonization of both Tuber species significantly increased leaf photosynthetic rate, leaf P concentration and mycorrhizosphere acid phosphatase activity, as well as richness of mycorrhizosphere bacterial communities of C. rockii seedlings. However, T. indicum colonization on the one hand significantly decreased tartrate content, bacterial acid phosphatase, phoC gene abundance in the mycorrhizosphere, and peroxidase (POD) activity of ectomycorrhizal root tips, but on the other hand increased mycorrhizosphere pH and superoxide dismutase (SOD) of ectomycorrhizal root tips, compared to T. lijiangense colonization. Moreover, principal coordinate and β-diversity analyses show significant differences in mycorrhizosphere bacterial community composition between T. indicum and T. lijiangese colonized C. rockii seedlings. Finally, the relative abundance of the bacterium Agromyces cerinus significantly correlated to mycorrhizosphere acid phosphatase activity and leaf P concentration, suggesting that this bacterium might play an important role in P mobilization and acquisition. Overall, these results suggest that T. indicum and T. lijiangense differently regulate their host plant's physiological responses and mycorrhizosphere bacterial community.

Keywords: ectomycorrhiza; mycorrhizosphere microbiome; nutrient acquisition; phoC and phoD; phosphatase activity; rhizosphere exudates.

Grants and funding

This work was funded by the National Key R&D Program of China (2021YFD1600404), Yunnan High Level Talent Introduction Plans to YW and XH, Yunnan Key Project of Science and Technology (202102AE090034), and the Basic Research Program of Yunnan Province (202101AU070080) as well as National Natural Science Foundation of China (32170302) to YW.