Responses of differential metabolites and pathways to high temperature in cucumber anther

Lin Chen; Zhaojun Liang; Shuyan Xie; Wenrui Liu; Min Wang; Jinqiang Yan; Songguang Yang; Biao Jiang; Qingwu Peng; Yu'e Lin

doi:10.3389/fpls.2023.1131735

Responses of differential metabolites and pathways to high temperature in cucumber anther

Front Plant Sci. 2023 Apr 14:14:1131735. doi: 10.3389/fpls.2023.1131735. eCollection 2023.

Authors

Lin Chen^{1

2}, Zhaojun Liang^{1

2}, Shuyan Xie^{1

2}, Wenrui Liu^{1

2}, Min Wang^{1

2}, Jinqiang Yan^{1

2}, Songguang Yang^{1

2}, Biao Jiang^{1

2}, Qingwu Peng^{1

2}, Yu'e Lin^{1

2}

Affiliations

¹ Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
² Guangdong Key Laboratory for New Technology Research of Vegetables, Guangzhou, China.

Abstract

Cucumber is one of the most important vegetable crops, which is widely planted all over the world. Cucumber always suffers from high-temperature stress in South China in summer. In this study, liquid chromatography-mass spectrometry (LC-MS) analysis was used to study the differential metabolites of cucumber anther between high-temperature (HT) stress and normal condition (CK). After HT, the pollen fertility was significantly reduced, and abnormal anther structures were observed by the paraffin section. In addition, the metabolomics analysis results showed that a total of 125 differential metabolites were identified after HT, consisting of 99 significantly upregulated and 26 significantly downregulated metabolites. Among these differential metabolites, a total of 26 related metabolic pathways were found, and four pathways showed significant differences, namely, porphyrin and chlorophyll metabolism; plant hormone signal transduction; amino sugar and nucleotide sugar metabolism; and glycine, serine, and threonine metabolism. In addition, pollen fertility was decreased by altering the metabolites of plant hormone signal transduction and amino acid and sugar metabolism pathway under HT. These results provide a comprehensive understanding of the metabolic changes in cucumber anther under HT.

Keywords: cucumber; high temperature stress; metabolomics; pollen fertility; starch and sucrose metabolism.

Grants and funding

This work was supported by the Guangdong Basic and Applied Basic Research Foundation (2022A1515011894), Key-Area Research and Development Program of Guangdong Province (2020B020220001, 2022B0202110003), National Science Foundation of China (32202477), Agricultural Competitive Industry Discipline Team Building Project of Guangdong Academy of Agricultural Sciences (202103TD), Special Fund for Scientific Innovation Strategy-Construction of High Level Academy of Agriculture Science (R2019YJ-YB3004), and Guangzhou Science and Technology Planning Project (202102020503).