Bioprinting and biomaterials for dental alveolar tissue regeneration

Serge Ostrovidov; Murugan Ramalingam; Hojae Bae; Gorka Orive; Toshinori Fujie; Xuetao Shi; Hirokazu Kaji

doi:10.3389/fbioe.2023.991821

Bioprinting and biomaterials for dental alveolar tissue regeneration

Front Bioeng Biotechnol. 2023 Apr 14:11:991821. doi: 10.3389/fbioe.2023.991821. eCollection 2023.

Authors

Serge Ostrovidov¹, Murugan Ramalingam^{2

3

4

5

6

7}, Hojae Bae⁸, Gorka Orive^{9

10

11}, Toshinori Fujie^{12

13}, Xuetao Shi¹⁴, Hirokazu Kaji¹

Affiliations

¹ Department of Biomechanics, Institute of Biomaterials and Bioengineering (IBB), Tokyo Medical and Dental University (TMDU), Tokyo, Japan.
² Institute of Tissue Regeneration Engineering, Dankook University, Cheonan, Republic of Korea.
³ Department of Nanobiomedical Science, BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, Republic of Korea.
⁴ School of Basic Medical Science, Chengdu University, Chengdu, China.
⁵ Mechanobiology Dental Medicine Research Center, Dankook University, Cheonan, Republic of Korea.
⁶ UCL Eastman-Korea Dental Medicine Innovation Centre, Dankook University, Cheonan, Republic of Korea.
⁷ Department of Metallurgical and Materials Engineering, Faculty of Engineering, Atilim University, Ankara, Türkiye.
⁸ Department of Stem Cell and Regenerative Biotechnology, KU Convergence Science and Technology Institute, Konkuk University, Hwayang-dong, Seoul, Republic of Korea.
⁹ NanoBioCel Group, Laboratory of Pharmaceutics, School of Pharmacy, University of the Basque Country UPV/EHU, Vitoria-Gasteiz, Spain.
¹⁰ Bioaraba, NanoBioCel Research Group, Vitoria-Gasteiz, Spain.
¹¹ Biomedical Research Networking Centre in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Vitoria-Gasteiz, Spain.
¹² School of Life Science and Technology, Tokyo Institute of Technology, Yokohama, Japan.
¹³ Living System Materialogy (LiSM) Reseach Group, International Research Frontiers Initiative (IRFI), Tokyo Institute of Technology, Yokohama, Japan.
¹⁴ National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, Guangdong, China.

Abstract

Three dimensional (3D) bioprinting is a powerful tool, that was recently applied to tissue engineering. This technique allows the precise deposition of cells encapsulated in supportive bioinks to fabricate complex scaffolds, which are used to repair targeted tissues. Here, we review the recent developments in the application of 3D bioprinting to dental tissue engineering. These tissues, including teeth, periodontal ligament, alveolar bones, and dental pulp, present cell types and mechanical properties with great heterogeneity, which is challenging to reproduce in vitro. After highlighting the different bioprinting methods used in regenerative dentistry, we reviewed the great variety of bioink formulations and their effects on cells, which have been established to support the development of these tissues. We discussed the different advances achieved in the fabrication of each dental tissue to provide an overview of the current state of the methods. We conclude with the remaining challenges and future needs.

Keywords: bioink; biomaterials; bioprinting; dental tissue engineering; dentistry.

Publication types

Review

Grants and funding

This work was supported by the Japan Society for the Promotion of Science (JSPS) KAKENHI (Grant Numbers 22K18936 and 21K04852); AMED (Grant Number JP21gm1310001); The JST Adaptable and Seamless Technology Transfer Program through Target-driven R&D (Grant Number JPMJTM22BD), CASIO SCIENCE PROMOTION FOUNDATION, and by the Research Center for Biomedical Engineering at Tokyo Medical and Dental University, Japan.