Ultrasensitive lateral flow biosensor based on PtAu@CNTs nanocomposite catalytic chromogenic signal amplification strategy for the detection of nucleic acid

Dongqing Xu; Xue An; Yuying Wang; Lisheng Qian; Wanwei Qiu; Xueji Zhang; Guodong Liu

doi:10.1016/j.aca.2023.341205

Ultrasensitive lateral flow biosensor based on PtAu@CNTs nanocomposite catalytic chromogenic signal amplification strategy for the detection of nucleic acid

Anal Chim Acta. 2023 Jun 15:1260:341205. doi: 10.1016/j.aca.2023.341205. Epub 2023 Apr 12.

Authors

Dongqing Xu¹, Xue An¹, Yuying Wang¹, Lisheng Qian¹, Wanwei Qiu², Xueji Zhang³, Guodong Liu⁴

Affiliations

¹ Institute of Biomedical and Health Science, School of Life and Health Science, Anhui Science and Technology University, Fengyang, 233100, Anhui, China.
² Institute of Biomedical and Health Science, School of Life and Health Science, Anhui Science and Technology University, Fengyang, 233100, Anhui, China. Electronic address: qiuwanweiqww@126.com.
³ Institute of Biomedical and Health Science, School of Life and Health Science, Anhui Science and Technology University, Fengyang, 233100, Anhui, China; School of Biomedical Engineering, Shenzhen University Healthy Science Center, Shenzhen, 518060, Guangdong, China. Electronic address: zhangxueji@ustb.edu.cn.
⁴ Institute of Biomedical and Health Science, School of Life and Health Science, Anhui Science and Technology University, Fengyang, 233100, Anhui, China; Department of Chemistry and Biochemistry, North Dakota State University, Fargo, 58105, ND, USA. Electronic address: guodong.liu@ndsu.edu.

PMID: 37121654
DOI: 10.1016/j.aca.2023.341205

Abstract

A rapid and ultrasensitive lateral flow biosensor was developed, which based on gold and platinum nanoparticles-decorated carbon nanotubes (PtAu@CNTs) nanocomposite catalytic chromogenic signal amplification strategy for the detection of nucleic acid. Independent platinum and gold nanoparticles modified functional carbon nanotubes (PtAu@CNTs) were prepared by in-situ reduction. Sandwich-type hybridization reaction occurred between PtAu@CNTs-labeled DNA probe, target DNA and Biotin-modified DNA probes, which was captured on test zone of the strip. Accumulation of PtAu@CNTs nano-labels formed a characteristic colored band. After systematic optimization and catalytic chromogen, the naked eye detection limit of PtAu@CNTs-LFA was about 2 pM, and the theoretical detection limit of target DNA is calculated to be 0.43 pM according to the standard curve. The results indicates a rapid, sensitive and specific methods for DNA detection in biological samples, showing great promise for biomedical diagnosis in some malignant diseases in clinical application.

Keywords: Catalytic chromogen; DNA detection; Lateral flow assay; PtAu@CNTs.

MeSH terms

Biosensing Techniques* / methods
DNA / genetics
DNA Probes / genetics
Gold
Limit of Detection
Metal Nanoparticles*
Nanotubes, Carbon*
Nucleic Acids*
Platinum

Substances

Nucleic Acids
Nanotubes, Carbon
Gold
Platinum
DNA
DNA Probes