Production of Designer Xylose-Acetic Acid Enriched Hydrolysate from Bioenergy Sorghum, Oilcane, and Energycane Bagasses

Ming-Hsun Cheng; Shuchi Singh; Aidan N Carr Clennon; Bruce S Dien; Vijay Singh

doi:10.1016/j.biortech.2023.129104

Production of Designer Xylose-Acetic Acid Enriched Hydrolysate from Bioenergy Sorghum, Oilcane, and Energycane Bagasses

Bioresour Technol. 2023 Jul:380:129104. doi: 10.1016/j.biortech.2023.129104. Epub 2023 Apr 28.

Authors

Ming-Hsun Cheng¹, Shuchi Singh², Aidan N Carr Clennon³, Bruce S Dien⁴, Vijay Singh⁵

Affiliations

¹ Department of Agricultural and Biological Engineering, University of Illinois Urbana-Champaign, Urbana IL 61801, USA; Department of Natural Resources and Society, College of Natural Resources, University of Idaho, 995 MK Simpson Blvd, Idaho Falls, ID 83401, USA; Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
² Department of Agricultural and Biological Engineering, University of Illinois Urbana-Champaign, Urbana IL 61801, USA; Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
³ Department of Agricultural and Biological Engineering, University of Illinois Urbana-Champaign, Urbana IL 61801, USA.
⁴ Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; United States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Center for Agricultural Utilization Research (NCAUR), Bioenergy Research Unit, 1815 North University Street, Peoria, IL 61604, USA.
⁵ Department of Agricultural and Biological Engineering, University of Illinois Urbana-Champaign, Urbana IL 61801, USA; Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. Electronic address: vsingh@illinois.edu.

PMID: 37121520
DOI: 10.1016/j.biortech.2023.129104

Abstract

Xylan accounts for up to 40% of the structural carbohydrates in lignocellulosic feedstocks. Along with xylan, acetic acid in sources of hemicellulose can be recovered and marketed as a commodity chemical. Through vibrant bioprocessing innovations, converting xylose and acetic acid into high-value bioproducts via microbial cultures improves the feasibility of lignocellulosic biorefineries. Enzymatic hydrolysis using xylanase supplemented with acetylxylan esterase (AXE) was applied to prepare xylose-acetic acid enriched hydrolysates from bioenergy sorghum, oilcane, or energycane using sequential hydrothermal-mechanical pretreatment. Various biomass solids contents (15 to 25%, w/v) and xylanase loadings (140 to 280 FXU/g biomass) were tested to maximize xylose and acetic acid titers. The xylose and acetic acid yields were significantly improved by supplementing with AXE. The optimal yields of xylose and acetic acid were 92.29% and 62.26% obtained from hydrolyzing energycane and oilcane at 25% and 15% w/v biomass solids using 280 FXU xylanase/g biomass and AXE, respectively.

Keywords: Acetic acid; Acetylxylan esterase; Sequential hydrothermal-mechanical pretreatment; Xylanase; Xylose.

MeSH terms

Acetic Acid
Hydrolysis
Sorghum*
Xylans
Xylose* / chemistry

Substances

Xylose
bagasse
Acetic Acid
Xylans