Metal coordinating-induced self-assembly of cyclic lipopeptides into high-performance antimicrobial supramolecules

Jinglin Ding; Shufan Zeng; Yueqing Wang; Xiaoyu Yin; Bo Zhang; Bolin Zhang; Shandong Xu; Yanyan Zhang; Jiangfu Zheng; Junfeng Fan; Mengze Wang

doi:10.1016/j.foodchem.2023.136203

Metal coordinating-induced self-assembly of cyclic lipopeptides into high-performance antimicrobial supramolecules

Food Chem. 2023 Oct 1:422:136203. doi: 10.1016/j.foodchem.2023.136203. Epub 2023 Apr 20.

Authors

Jinglin Ding¹, Shufan Zeng¹, Yueqing Wang¹, Xiaoyu Yin¹, Bo Zhang¹, Bolin Zhang¹, Shandong Xu², Yanyan Zhang³, Jiangfu Zheng⁴, Junfeng Fan⁵, Mengze Wang⁶

Affiliations

¹ College of Biological Sciences and Technology, Beijing Key Laboratory of Food Processing and Safety in Forestry, Beijing Forestry University, Beijing 100083, China.
² College of Science, Beijing Forestry University, Beijing 100083, China.
³ Food Science and Engineering College, Beijing University of Agriculture, Beijing 102206, China.
⁴ College of Environmental Science and Engineering, Hunan University, Changsha 410082, China.
⁵ College of Biological Sciences and Technology, Beijing Key Laboratory of Food Processing and Safety in Forestry, Beijing Forestry University, Beijing 100083, China. Electronic address: fanjunfeng@bjfu.edu.cn.
⁶ School of Food & Wine, Ningxia University, Yinchuan 750021, China. Electronic address: wangmengze@nxu.edu.cn.

PMID: 37121207
DOI: 10.1016/j.foodchem.2023.136203

Abstract

This study designed a green hydrothermally-chelating approach to generate robust antimicrobial complexes via metal-coordinated supramolecular self-assembly of cyclic lipopeptides (CLs). The metal ion (Ca²⁺ and Zn²⁺)-coordinated CL (Ca/CL or Zn/CL complex; 1 mg/mL) demonstrated potent antibacterial activity against fungi (A. niger) and bacteria (E. coli and S. aureus) respectively, and in particular, completely suppressed the microbial resistance. Further physicochemical and spectal analysis showed that this coordination approach led to CL with enhanced hydrophobic and intermolecular electrostatic interactions, forming β-sheet-rich secondary structures allowing the complexes easily contact with and destroy the membrane of microorganisms. Practical application experiments validated that the Ca/CL and Zn/CL complexes strongly avoided table grape and fresh tomato from the contamination of pathogen. The findings of this study laid foundation for the utilization of metal ions to improve the biological activity of natural antimicrobial peptides.

Keywords: Antimicrobial peptides; Bacillus subtilis; Metal coordination.

MeSH terms

Anti-Infective Agents* / pharmacology
Coordination Complexes* / chemistry
Escherichia coli
Lipopeptides / pharmacology
Metals / chemistry
Microbial Sensitivity Tests
Staphylococcus aureus

Substances

Anti-Infective Agents
Metals
Lipopeptides
Coordination Complexes