Detection of CD36 gene polymorphism associated with chicken carcass traits and skin yellowness

Changbin Zhao; Bowen Hu; Ze Zhang; Qingbin Luo; Qinghua Nie; Xiquan Zhang; Hongmei Li

doi:10.1016/j.psj.2023.102691

Detection of CD36 gene polymorphism associated with chicken carcass traits and skin yellowness

Poult Sci. 2023 Jul;102(7):102691. doi: 10.1016/j.psj.2023.102691. Epub 2023 Apr 4.

Authors

Changbin Zhao¹, Bowen Hu¹, Ze Zhang¹, Qingbin Luo¹, Qinghua Nie¹, Xiquan Zhang¹, Hongmei Li²

Affiliations

¹ State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Lingnan Guangdong Laboratory of Agriculture, College of Animal Science, South China Agricultural University, Guangzhou 510642, China; National-Local Joint Engineering Research Center for Livestock Breeding, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, and Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, China.
² State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Lingnan Guangdong Laboratory of Agriculture, College of Animal Science, South China Agricultural University, Guangzhou 510642, China; National-Local Joint Engineering Research Center for Livestock Breeding, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, and Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, China. Electronic address: hongmeili@scau.edu.cn.

Abstract

Investigations into the association between chicken traits and genetic variations provide helpful breeding information to improve production performance and economic benefits in chickens. The single nucleotide polymorphism technique is an important method in agricultural molecular breeding. In this study, we detected 11 SNPs in the CD36 gene, 2 SNPs (g.-1974 A>G, g.-1888 T>C) located in the 5' flanking regions, 8 SNPs (g.23496 G>A, g.23643 C>T, g.23931 T>C, g.23937 G>A, g.31256 C>A, g.31258 C>T, g.31335 C>T, g.31534 A>C) located in the intron region, 1 SNPs (g.23743 G>T) located in the exon region and it belongs to synonymous mutation. In SNPs g.23743 G>T, the abdominal fat weight and abdominal fat weight rate of the GG genotype were lower than that of the TT genotype. In SNPs g.23931 T>C, the full-bore weight rate and half-bore weight rate of the TT genotype were higher compared with the CC genotype. And the SNPs g.-1888 T>C, g.23496 G>A, g.23643 C>T, g.31335 C>T and g.31534 A>C were significantly associated with skin yellowness traits, the cloacal skin yellowness before slaughter of the TT genotype was higher than that of the TC and CC genotype in SNPs g.-1888 T>C. Furthermore, 3 haplotypes of the above eleven SNPs were calculated and they correlated with heart weight, stomach weight, wing weight, leg skin yellowness and shin skin yellowness before slaughter. Finally, the CD36 expression profile displayed the expression pattern of CD36 mRNA variation in different tissues.

Keywords: CD36; carcass trait; chicken; single nucleotide polymorphism; skin yellowness.

MeSH terms

Animals
Chickens* / genetics
Genotype
Phenotype
Polymorphism, Single Nucleotide
Quantitative Trait, Heritable*