Data on cloning, expression and biochemical characteristics of a chondroitin sulfate/dermatan sulfate 4- O-endosulfatase

Lin Wei; Yingying Xu; Min Du; Ying Fan; Ruyi Zou; Xiangyu Xu; Qingdong Zhang; Yu-Zhong Zhang; Wenshuang Wang; Fuchuan Li

doi:10.1016/j.dib.2023.109139

Data on cloning, expression and biochemical characteristics of a chondroitin sulfate/dermatan sulfate 4- O-endosulfatase

Data Brief. 2023 Apr 10:48:109139. doi: 10.1016/j.dib.2023.109139. eCollection 2023 Jun.

Authors

Lin Wei¹, Yingying Xu¹, Min Du¹, Ying Fan², Ruyi Zou¹, Xiangyu Xu¹, Qingdong Zhang³, Yu-Zhong Zhang⁴, Wenshuang Wang¹, Fuchuan Li^{1

4}

Affiliations

¹ National Glycoengineering Research Center, Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology and State Key Laboratory of Microbial Technology, Shandong University, 72 Binhai Rd, Qingdao 266237, China.
² Qingdao Special Servicemen Recuperation Center of PLA Navy, Qingdao 266071, China.
³ School of Life Science and Technology, Weifang Medical University, 7166 Baotong West Street, Weifang 261053, China.
⁴ College of Marine Life Sciences, Ocean University of China, Qingdao, China.

Abstract

The data shown in this article are related to the published paper entitled "A novel 4-O-endosulfatase with high potential for the structure-function studies of chondroitin sulfate/dermatan sulfate" in Carbohydrate Polymers. In this article, the phylogenetic analysis, cloning, expression, purification, specificity and biochemical characteristics of the identified chondroitin sulfate/dermatan sulfate 4-O-endosulfatase (endoBI4SF) are described in detail. The recombinant endoBI4SF with a molecular mass of 59.13 kDa can can specifically hydrolyze the 4-O- but not 2-O- and 6-O-sulfate groups in the oligo-/polysaccharides of chondroitin sulfate/dermatan sulfate and show the maximum reaction rate in 50 mM Tris-HCl buffer (pH 7.0) at 50°C, which can be a very useful tool for the structural and functional studies of chondroitin sulfate/dermatan sulfate.

Keywords: Biochemical characteristics; Cloning and expression; Substrate specificity; Sulfatase.