A Rice Receptor-like Protein Negatively Regulates Rice Resistance to Southern Rice Black-Streaked Dwarf Virus Infection

Fengmin Wang; Weiqi Song; Chaorui Huang; Zhongyan Wei; Yanjun Li; Jianping Chen; Hehong Zhang; Zongtao Sun

doi:10.3390/v15040973

A Rice Receptor-like Protein Negatively Regulates Rice Resistance to Southern Rice Black-Streaked Dwarf Virus Infection

Viruses. 2023 Apr 15;15(4):973. doi: 10.3390/v15040973.

Authors

Fengmin Wang¹, Weiqi Song¹, Chaorui Huang¹, Zhongyan Wei¹, Yanjun Li¹, Jianping Chen¹, Hehong Zhang¹, Zongtao Sun¹

Affiliation

¹ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of MOA of China and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, China.

Abstract

Plants rely on various receptor-like proteins and receptor-like kinases to recognize and defend against invading pathogens. However, research on the role of receptor-like proteins in plant antiviral defense, particularly in rice-virus interactions, is limited. In this study, we identified a receptor-like gene, OsBAP1, which was significantly induced upon infection with southern rice black-streaked dwarf virus (SRBSDV) infection. A viral inoculation assay showed that the OsBAP1 knockout mutant exhibited enhanced resistance to SRBSDV infection, indicating that OsBAP1 plays a negatively regulated role in rice resistance to viral infection. Transcriptome analysis revealed that the genes involved in plant-pathogen interactions, plant hormone signal transduction, oxidation-reduction reactions, and protein phosphorylation pathways were significantly enriched in OsBAP1 mutant plants (osbap1-cas). Quantitative real-time PCR (RT-qPCR) analysis further demonstrated that some defense-related genes were significantly induced during SRBSDV infection in osbap1-cas mutants. Our findings provide new insights into the role of receptor-like proteins in plant immune signaling pathways, and demonstrate that OsBAP1 negatively regulates rice resistance to SRBSDV infection.

Keywords: pattern-triggered immunity; receptor-like protein; rice; southern rice black-streaked dwarf virus; transcriptome.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Gene Expression Profiling
Oryza* / genetics
Plant Diseases
Reoviridae* / genetics
Viral Proteins / genetics

Substances

Viral Proteins

Supplementary concepts

Southern rice black-streaked dwarf virus

Grants and funding

This work was funded by the National Natural Science Foundation of China (32270149 and 32272555) and the Young Elite Scientist Sponsorship Program by CAST (YESS20210121). This work was sponsored by the K. C. Wong Magna Fund in Ningbo University.