Interactions of Tissue-Resident T Cells

Rut Mora-Buch; Hasan Akbaba; Shannon K Bromley

doi:10.1007/978-1-0716-3135-5_28

Interactions of Tissue-Resident T Cells

Methods Mol Biol. 2023:2654:437-452. doi: 10.1007/978-1-0716-3135-5_28.

Authors

Rut Mora-Buch^{1

2}, Hasan Akbaba^{1

3}, Shannon K Bromley⁴

Affiliations

¹ Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy and Immunology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
² Cell Therapy Services, Blood and Tissue Bank, Barcelona, Spain.
³ Department of Pharmaceutical Biotechnology Faculty of Pharmacy, Ege University, Bornova, Izmir, Turkey.
⁴ Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy and Immunology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA. sbromley@mgh.harvard.edu.

PMID: 37106199
DOI: 10.1007/978-1-0716-3135-5_28

Abstract

Resident memory T cells (T_RM) are non-circulating cells that play a critical role in protection from local infections and cancers. Flow cytometric and transcriptional analyses of these cells have defined their distinct phenotypes; imaging allows study of their morphology, localization, and interactions within tissues. Here, we describe commonly used methods to generate cutaneous CD8⁺ T_RM and to prepare skin samples for analysis, including staining of cryostat sections, epidermal sheets, and tissue whole mounts.

Keywords: Epidermal sheets; Immunofluorescence microscopy; Resident memory CD8+ T cells; Skin; TRM; Whole mount.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

CD8-Positive T-Lymphocytes*
Epidermis
Immunologic Memory
Skin*

Grants and funding

R01 AI163517/AI/NIAID NIH HHS/United States