Quantification of hetero-cyclic amines from different categories of braised beef by optimized UPLC-TQ-XS/ESI method

Zahra Batool; Wang Xia; Jie-Hua Chen; Yuge Bi; Feng Chen; Mingfu Wang

doi:10.1016/j.foodchem.2023.136191

Quantification of hetero-cyclic amines from different categories of braised beef by optimized UPLC-TQ-XS/ESI method

Food Chem. 2023 Sep 30:421:136191. doi: 10.1016/j.foodchem.2023.136191. Epub 2023 Apr 19.

Authors

Zahra Batool¹, Wang Xia², Jie-Hua Chen², Yuge Bi², Feng Chen³, Mingfu Wang⁴

Affiliations

¹ College of Civil and Transportation Engineering, Shenzhen University, Shenzhen 518060, China; Institute for Innovative Development of Food Industry, Shenzhen University, Shenzhen 518060, China; Shenzhen Key Laboratory of Marine Microbiome Engineering, Institute for Advanced Study, Shenzhen University, Shenzhen 518060, China.
² Institute for Innovative Development of Food Industry, Shenzhen University, Shenzhen 518060, China; Shenzhen Key Laboratory of Marine Microbiome Engineering, Institute for Advanced Study, Shenzhen University, Shenzhen 518060, China.
³ Institute for Innovative Development of Food Industry, Shenzhen University, Shenzhen 518060, China; Shenzhen Key Laboratory of Marine Microbiome Engineering, Institute for Advanced Study, Shenzhen University, Shenzhen 518060, China. Electronic address: sfchen@szu.edu.cn.
⁴ Institute for Innovative Development of Food Industry, Shenzhen University, Shenzhen 518060, China; Shenzhen Key Laboratory of Marine Microbiome Engineering, Institute for Advanced Study, Shenzhen University, Shenzhen 518060, China. Electronic address: mfwang@szu.edu.cn.

PMID: 37105122
DOI: 10.1016/j.foodchem.2023.136191

Abstract

This research work has developed and optimized a sensitive analytical method for separation and quantification of heterocyclic amines (HCAs) mainly including PhIP, Harman, Norharman, IQ, MeIQ, AαC, MeAαC and Trp-P-2 by optimizing UPLC-TQ-XS using electrospray ionization source (ESI+) on ACQUITY UPLC® BEH C18 column in <7 min, from braised beef sample matrix. Meanwhile, modified HCAs extraction by modifying QuEChERS (quick, easy, cheap, efficient, rugged and safe) technique and revisited with solid phase extraction (SPE) for HCAs purification, instead using traditional QuEChERS salts. Moreover, optimized pH conditions of HCA extracts before purification, for better extraction recoveries. Furthermore, this method was validated in terms of method validation parameters. Lastly, simulation of real braised beef model provided the minimum formation of HCAs by optimizing cooking parameters and precursors in a cooking system. Therefore, this method could be applied simultaneously on braised beef matrix either marketed or home cooked for HCAs analysis.

Keywords: Analysis; Extraction; Heterocyclic amines; Quantification; Recovery; Solid phase extraction.

MeSH terms

Amines / analysis
Animals
Cattle
Chromatography, High Pressure Liquid
Cooking / methods
Heterocyclic Compounds* / analysis
Research Design
Solid Phase Extraction

Substances

Heterocyclic Compounds
Amines