Generation of two human NRF2 knockout iPSC clones using CRISPR/Cas9 editing

Sylvia Merkert; Alexandra Haase; Julia Dahlmann; Gudrun Göhring; Fakhar H Waqas; Frank Pessler; Ulrich Martin; Ruth Olmer

doi:10.1016/j.scr.2023.103090

Generation of two human NRF2 knockout iPSC clones using CRISPR/Cas9 editing

Stem Cell Res. 2023 Jun:69:103090. doi: 10.1016/j.scr.2023.103090. Epub 2023 Apr 1.

Authors

Sylvia Merkert¹, Alexandra Haase², Julia Dahlmann³, Gudrun Göhring⁴, Fakhar H Waqas⁵, Frank Pessler⁶, Ulrich Martin³, Ruth Olmer³

Affiliations

¹ Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, 30625 Hannover, Germany; Biomedical Research in Endstage and Obstructive Lung Disease (BREATH), Member of the German Center for Lung Research (DZL), Hannover Medical School, 30625 Hannover, Germany; REBIRTH-Research Center for Translational and Regenerative Medicine, Hannover Medical School, 30625 Hannover, Germany. Electronic address: merkert.sylvia@mh-hannover.de.
² Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, 30625 Hannover, Germany; REBIRTH-Research Center for Translational and Regenerative Medicine, Hannover Medical School, 30625 Hannover, Germany.
³ Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, 30625 Hannover, Germany; Biomedical Research in Endstage and Obstructive Lung Disease (BREATH), Member of the German Center for Lung Research (DZL), Hannover Medical School, 30625 Hannover, Germany; REBIRTH-Research Center for Translational and Regenerative Medicine, Hannover Medical School, 30625 Hannover, Germany.
⁴ Department of Human Genetics, Hannover Medical School, 30625 Hannover, Germany.
⁵ TWINCORE Centre for Experimental and Clinical Infection Research, 30625 Hannover, Germany; Helmholtz-Centre for Infection Research Braunschweig, 38124 Braunschweig, Germany.
⁶ TWINCORE Centre for Experimental and Clinical Infection Research, 30625 Hannover, Germany; Helmholtz-Centre for Infection Research Braunschweig, 38124 Braunschweig, Germany; Centre for Individualized Infection Medicine, 30625 Hannover, Germany.

PMID: 37104932
DOI: 10.1016/j.scr.2023.103090

Abstract

The nuclear factor erythroid 2-related factor 2 (NFE2L2, known as NRF2) regulates the expression of antioxidative and anti-inflammatory proteins. In order to investigate its impact during viral infections and testing of antiviral compounds, we applied CRISPR/Cas9 editing to eliminate NRF2 in the human iPS cell line MHHi001-A and generated two NRF2 knockout iPSC clones MHHi001-A-6 and MHHi001-A-7. After differentiation into epithelia or endothelial cells, these cells are useful tools to examine the antiviral effects of activators of the NRF2 signaling pathway.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CRISPR-Cas Systems* / genetics
Clone Cells / metabolism
Endothelial Cells / metabolism
Humans
Induced Pluripotent Stem Cells* / metabolism
NF-E2-Related Factor 2 / genetics
NF-E2-Related Factor 2 / metabolism

Substances

NF-E2-Related Factor 2