[Effect of P-coumaric Acid on Apoptosis of Multiple Myeloma Cells Based on Oxidative Stress]

Zhu-Fa Hou; Bing-Jie Zhao; Song-Shan Liu; Wen-Jing Yi; Hong Che

doi:10.19746/j.cnki.issn.1009-2137.2023.02.018

[Effect of P-coumaric Acid on Apoptosis of Multiple Myeloma Cells Based on Oxidative Stress]

Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2023 Apr;31(2):435-441. doi: 10.19746/j.cnki.issn.1009-2137.2023.02.018.

[Article in Chinese]

Authors

Zhu-Fa Hou¹, Bing-Jie Zhao², Song-Shan Liu³, Wen-Jing Yi¹, Hong Che¹

Affiliations

¹ Department of Hematology, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610072, Sichuan Province, China.
² Department of Hematology, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610072, Sichuan Province, China .E-mail: zhaobingjiedk @163.com.
³ Department of Hematology, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610072, Sichuan Province, China . E-mail: pine009@163.com.

PMID: 37096516
DOI: 10.19746/j.cnki.issn.1009-2137.2023.02.018

Abstract
in English, Chinese

Objective: To investigate the effect of p-coumaric acid on apoptosis of multiple myeloma cells and its related mechanism.

Methods: Multiple myeloma cell line MM.1s cells were selected and treated with different concentrations of p-coumaric acid (0, 0.4, 0.8, 1.6, 3.2 mmol/L), and the inhibition rate and half inhibition concentration (IC₅₀) were detected by CCK-8 method. Then MM.1s cells were treated with 1/2 IC₅₀, IC₅₀, 2 IC₅₀ and transfected with ov-Nrf-2 and ov-Nrf-2+IC₅₀. The apoptosis, ROS fluorescence intensity and mitochondrial membrane potential of MM.1s cells were detected by flow cytometry, and the relative expressions of cellular Nrf-2 and HO-1 protein were detected by Western blot.

Results: P-coumaric acid inhibited the proliferation of MM.1s cells in a dose-dependent manner(r =0.997) with an IC₅₀ value of 2.754 mmol/L. Compared with the control group, apoptosis and ROS fluorescence intensity of MM.1s cells were significantly increased in the 1/2 IC₅₀ group, IC₅₀ group, 2 IC₅₀ group and ov-Nrf-2+IC₅₀ group (P <0.01), the expressions of Nrf-2, HO-1 protein in the IC₅₀ group and 2 IC₅₀ group were significantly decreased (P <0.05). Compared with the IC₅₀ group, the cells apoptosis and ROS fluorescence intensity were significantly decreased (P <0.01), and the expressions of Nrf-2 and HO-1 protein were significantly increased in the ov-Nrf-2+IC₅₀ group (P <0.01).

Conclusion: P-coumaric acid can inhibit the proliferation of MM.1s cells and may target the Nrf-2/HO-1 signaling pathway to affect oxidative stress in MM cells thereby inducing their apoptosis.

题目: 基于氧化应激的对香豆酸对多发性骨髓瘤细胞凋亡的影响.

目的: 探讨对香豆酸对多发性骨髓瘤细胞凋亡的影响及相关作用机制。.

方法: 选择多发性骨髓瘤细胞MM.1s，以不同浓度对香豆酸（0、0.4、0.8、1.6、3.2 mmol/L）作用于MM.1s细胞，采用CCK-8法检测细胞抑制率及半数抑制浓度（IC₅₀）；并以1/2 IC₅₀、IC₅₀、2 IC₅₀及转染ov-Nrf-2、ov-Nrf-2+IC₅₀作用于MM.1s细胞，采用流式细胞术检测细胞凋亡、ROS荧光强度及线粒体膜电位；采用蛋白质印迹法（Western blot）检测细胞Nrf-2、HO-1蛋白相对表达量。.

结果: 对香豆酸以剂量依赖性方式抑制MM.1s细胞的增殖（r =0.997），其IC₅₀值为2.754 mmol/L；与对照组相比，1/2 IC₅₀组、IC₅₀组、2 IC₅₀组、ov-Nrf-2+IC₅₀组细胞凋亡及ROS荧光强度均明显增加（P <0.01），IC₅₀组、2 IC₅₀组Nrf-2、HO-1蛋白表达均明显降低（P <0.05）；与IC₅₀组相比，ov-Nrf-2+IC₅₀组细胞凋亡、ROS荧光强度明显降低（P <0.01），Nrf-2、HO-1蛋白表达明显升高（P <0.01）。.

结论: 对香豆酸能抑制MM.1s细胞增殖，可能靶向Nrf-2/HO-1信号通路影响MM细胞氧化应激从而诱导细胞凋亡。.

Keywords: multiple myeloma; Oxidative stress; P-coumaric acid; cell apoptosis.

Publication types

English Abstract

MeSH terms

Apoptosis
Cell Line, Tumor
Humans
Multiple Myeloma*
Oxidative Stress
Reactive Oxygen Species / metabolism
Reactive Oxygen Species / pharmacology

Substances

p-coumaric acid
Reactive Oxygen Species

Abstract in English, Chinese

Publication types

MeSH terms

Substances

Abstract
in English, Chinese