Gelation behaviors and mechanism of a new pectic polysaccharide from apple pomace as a potential gelatin substitute

Abstract

In this study, we reported a pectic polysaccharide industrially obtained from apple pomace by metal ion precipitation technique showing an unexpected gelation behavior. Structurally, this apple pectin (AP) is a macromolecular polymer with a weight-average molecular weight (Mw) of 361.7 kDa, and DM (degree of methoxylation) of 12.5 %, comprising 60.38 % glucose, 19.41 % mannose, 17.60 % galactose and 1.00 % rhamnose and 1.61 % glucuronic acid. The low acidic sugar percentage relative to the total monosaccharide amount indicated a high branching structure of AP. On addition of Ca²⁺ ions, AP exhibited a remarkable gelling ability upon cooling its heat solution to low temperature (e.g., 4 °C). However, at room temperature (e.g., 25 °C) or in the absence of Ca²⁺, no gel was formed. At a fixed pectin concentration (0.5 %, w/v), AP showed increasing gel hardness and gelation temperature (T_gel) with CaCl₂ concentration increasing to 0.05 % (w/v); however, further addition of CaCl₂ weakened AP gels and even abolished the gelation. On reheating, all gels melted below 35 °C, which suggests the potential use of AP as a gelatin substitute. The gelation mechanism was explained as an intricate balance of the synchronous formation of hydrogen bond and Ca²⁺ crosslinks between AP molecules during cooling.

Keywords: Apple pectin; Gelatin substitute; Gelation; Gels; Rheology.