Hyperpolarized NMR metabolomics

Victor Ribay; Clément Praud; Marine P M Letertre; Jean-Nicolas Dumez; Patrick Giraudeau

doi:10.1016/j.cbpa.2023.102307

Hyperpolarized NMR metabolomics

Curr Opin Chem Biol. 2023 Jun:74:102307. doi: 10.1016/j.cbpa.2023.102307. Epub 2023 Apr 23.

Authors

Victor Ribay¹, Clément Praud¹, Marine P M Letertre¹, Jean-Nicolas Dumez¹, Patrick Giraudeau²

Affiliations

¹ Nantes Université, CNRS, CEISAM UMR 6230, F-44000 Nantes, France.
² Nantes Université, CNRS, CEISAM UMR 6230, F-44000 Nantes, France. Electronic address: patrick.giraudeau@univ-nantes.fr.

PMID: 37094508
DOI: 10.1016/j.cbpa.2023.102307

Abstract

Hyperpolarized NMR is a promising approach to address the sensitivity limits of conventional NMR metabolomics approaches, which currently fails to detect minute metabolite concentrations in biological samples. This review describes how tremendous signal enhancement offered by dissolution-dynamic nuclear polarization and parahydrogen-based techniques can be fully exploited for molecular omics sciences. Recent developments, including the combination of hyperpolarization techniques with fast multi-dimensional NMR implementation and quantitative workflows are described, and a comprehensive comparison of existing hyperpolarization techniques is proposed. High-throughput, sensitivity, resolution and other relevant challenges that should be tackled for a general application of hyperpolarized NMR in metabolomics are discussed.

Keywords: Dissolution dynamic nuclear polarization; Hyperpolarization; Metabolomics; NMR spectroscopy; Para-hydrogen.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Magnetic Resonance Imaging*
Magnetic Resonance Spectroscopy / methods
Metabolomics* / methods