Complete genome sequence of functional probiotic candidate Lactobacillus amylovorus CACC736

Soyeon Park; Jung-Ae Kim; Hyun-Jun Jang; Dae-Hyuk Kim; Yangseon Kim

doi:10.5187/jast.2022.e85

Complete genome sequence of functional probiotic candidate Lactobacillus amylovorus CACC736

J Anim Sci Technol. 2023 Mar;65(2):473-477. doi: 10.5187/jast.2022.e85. Epub 2023 Mar 31.

Authors

Soyeon Park¹, Jung-Ae Kim^{1

2}, Hyun-Jun Jang¹, Dae-Hyuk Kim^{1

3}, Yangseon Kim¹

Affiliations

¹ Department of Research and Development, Center for Industrializaton of Agricultural and Livestock Microorganisms, Jeongeup 56212, Korea.
² Department of Bioactive Material Science, Jeonbuk National University, Jeonju 54896, Korea.
³ Department of Molecular Biology, Department of Bioactive Material Science, Institute for Molecular Biology and Genetics, Jeonbuk National University, Jeonju 54896, Korea.

Abstract

Lactobacillus amylovorus CACC736 was originated from swine feces in Korea. The complete genome sequences of the strain contained one circular chromosome (2,057,809 base pair [bp]) with 38.2% guanine-cytosine (GC) content and two circular plasmids, namely, pCACC736-1 and pCACC736-2. The predicted protein-coding genes, which are encoding the clustered regularly interspaced short palindromic repeats (CRISPR)-associated proteins, biosynthesis of bacteriocin (helveticin J), and the related proteins of the bile, acid tolerance. Notably, the genes related to vitamin B-group biosynthesis (riboflavin and cobalamin) were also found in L. amylovorus CACC736. Collectively, the complete genome sequence of the L. amylovorus CACC736 will aid in the development of functional probiotics in the animal industry.

Keywords: Lactobacillus amylovorus; Probiotics; Swine; Whole-genome sequencing.